β-Lactoglobulin Is Insulinotropic Compared with Casein and Whey Protein Ingestion during Catabolic Conditions in Men in a Double-Blinded Randomized Crossover Trial
| Autor: | Britt Christensen, Bolette Hartmann, Niels Møller, Elin Rakvaag, Maike Mose, Jens Otto Lunde Jørgensen, Niels Jessen, Ulla Ramer Mikkelsen, Nikolaj Rittig, Jens J. Holst |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Whey protein fasting Phenylalanine medicine.medical_treatment Muscle Proteins Medicine (miscellaneous) Gastric Inhibitory Polypeptide Lactoglobulins Young Adult Gastric inhibitory polypeptide Double-Blind Method Casein Internal medicine medicine Humans Ingestion skeletal muscle dairy protein Cross-Over Studies Nutrition and Dietetics endotoxemia business.industry Insulin Caseins Skeletal muscle Crossover study Whey Proteins Endocrinology medicine.anatomical_structure immobilization Bolus (digestion) business |
| Zdroj: | Mose, M, Møller, N, Jessen, N, Mikkelsen, U R, Christensen, B, Rakvaag, E, Hartmann, B, Holst, J J, Jørgensen, J O L & Rittig, N 2021, ' β-Lactoglobulin Is Insulinotropic Compared with Casein and Whey Protein Ingestion during Catabolic Conditions in Men in a Double-Blinded Randomized Crossover Trial ', The Journal of Nutrition, vol. 151, no. 6, pp. 1462-1472 . https://doi.org/10.1093/jn/nxab010 |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/nxab010 |
| Popis: | BACKGROUND: Muscle loss during acute infectious disease is mainly triggered by inflammation, immobilization, and malnutrition.OBJECTIVE: The objective was to compare muscle protein kinetics and metabolism following ingestion of the dairy protein supplements β-lactoglobulin (BLG), casein (CAS), and whey (WHE) during controlled catabolic conditions.METHODS: We used a randomized crossover design (registered at clinicaltrials.gov as NCT03319550) to investigate 9 healthy male participants [age: 20-40 y; BMI (in kg/m2) 20-30] who were randomly assigned servings of BLG, CAS, or WHE (0.6 g protein/kg, one-third as bolus and two-thirds as sip every 20 min) on 3 separate occasions separated by ∼6-8 wk. The participants received an infusion of lipopolysaccharide (1 ng/kg) combined with 36 h of fasting and bed rest before each study day, mimicking a clinical catabolic condition. The forearm model and isotopic tracer techniques were used to quantify muscle protein kinetics. Muscle biopsy specimens were obtained and intramyocellular signaling investigated using Western blot.RESULTS: BLG, CAS, and WHE improved the net balance of phenylalanine (NBphe) from baseline with ∼75% (P < 0.001) with no difference between interventions (primary outcome, P < 0.05). No difference in rates of appearance and disappearance of phenylalanine or in intramyocellular signaling activation was found between interventions (secondary outcomes). The incremental AUC for serum insulin was 62% higher following BLG compared with CAS (P < 0.001) and 30% higher compared with WHE (P = 0.002), as well as 25% higher in WHE compared with CAS (P = 0.006). Following BLG consumption, plasma concentrations of glucose-dependent insulinotropic peptide (GIP) increased 70% compared with CAS (P = 0.001) and increased 34% compared with WHE (P = 0.06). No significant difference was found between WHE and CAS (P = 0.12).CONCLUSION: BLG, WHE, and CAS have similar effects on muscle in young male participants during catabolic conditions. BLG showed specific, possibly GIP-dependent, insulinotropic properties, which may have future clinical implications. |
| Databáze: | OpenAIRE |
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