Acceleration of tendon healing using US guided intratendinous injection of bevacizumab: first pre-clinical study on a murine model
| Autor: | Lydia Deschamps, Ahmed Larbi, Yohan Benayoun, Agathe Hess, Liliane Louedec, Jean-Baptiste Michel, Benjamin Dallaudière, Jean Michel Serfaty, P Meyer, Lionel Pesquer, Pierre-Marie Preux, Clément Journé, E. Schouman-Claeys, Marta Lempicki, Vincent Hummel, Anne Perozziello, Marie Hélène Moreau Durieux |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Bevacizumab medicine.drug_class Joint mobilization Tendinosis Urology Angiogenesis Inhibitors Pilot Projects Injections Intralesional Antibodies Monoclonal Humanized Rats Sprague-Dawley medicine Animals Humans Radiology Nuclear Medicine and imaging Collagenases Ultrasonography Interventional Wound Healing business.industry Histology General Medicine Recovery of Function musculoskeletal system medicine.disease Surgery Tendon Rats Disease Models Animal medicine.anatomical_structure Treatment Outcome Toxicity Tendinopathy Corticosteroid business medicine.drug |
| Zdroj: | European journal of radiology. 82(12) |
| ISSN: | 1872-7727 |
| Popis: | Tendinopathy shows early disorganized collagen fibers with neo-angiogenesis on histology. Peri-tendinous injection of corticosteroid is the commonly accepted strategy despite the abscence of inflammation in tendinosis. The aim of our study was to assess the potential of intratendinous injection of an anti-angiogenic drug (bevacizumab, AA) to treat tendinopathy in a murine model of patellar and Achilles tendinopathy, and to evaluate its local toxicity.Forty rats (160 patellar and Achilles tendons) were used for this study. We induced tendinosis (T+) in 80 tendons by injecting under ultrasonography (US) guidance Collagenase 1(®) (day 0 = D0, patellar = 40 and Achilles = 40). Clinical examination and tendon US were performed at D3, immediately followed by either AA (AAT+, n = 40) or physiological serum (PST+, n = 40, control) US-guided intratendinous injection. Follow-up at D6 and D13 using clinical, US and histology, and comparison between the 2 groups were performed. To study AA toxicity we compared the 80 remaining normal tendons (T-) after injecting AA in 40 (AAT-).All AAT+ showed a better joint mobilization compared to PST+ at D6 (p = 0.004) with thinner US tendon diameters (p0.004), and less disorganized collagen fibers and neovessels on histology (p0.05). There was no difference at D13 regarding clinical status, US tendon diameter and histology (p0.05). Comparison between AAT- and T- showed no AA toxicity on tendon (p = 0.18).Our study suggests that high dose mono-injection of AA in tendinosis, early after the beginning of the disease, accelerates tendon's healing, with no local toxicity. |
| Databáze: | OpenAIRE |
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