Single channel analysis of ketamine interaction with a quisqualate receptor
| Autor: | Alister Macdonald, C.J. Shelton, Peter N.R. Usherwood, R. L. Ramsey |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Glutamic Acid AMPA receptor Grasshoppers Membrane Potentials Glutamates Internal medicine medicine Animals Ketamine Patch clamp Receptors AMPA General anaesthetic Pharmacology biology Dose-Response Relationship Drug Muscles biology.organism_classification Electric Stimulation Receptors Neurotransmitter Electrophysiology Kinetics Endocrinology Biophysics Female Channel analysis Ion Channel Gating Locust Communication channel medicine.drug |
| Zdroj: | European journal of pharmacology. 210(3) |
| ISSN: | 0014-2999 |
| Popis: | The interactions of the general anaesthetic ketamine with the quisqualate-sensitive L-glutamate receptor (QUIS-R) of locust muscle have been investigated at the single channel level using a M omega seal patch clamp technique. Low concentrations (10(-10) to 10(-9) M) of ketamine did not significantly alter the kinetics of the QUIS-R channel. Higher concentrations of ketamine decreased the probability of the channel being open, the frequency of channel opening and the channel mean open time, and increased the channel mean closed time. Probability density functions of channel dwell times indicate that during application of greater than 10(-8) M ketamine the distribution of channel openings becomes restricted mainly to brief events. These results are consistent with the view that ketamine blocks the open, and possibly also the closed, channel of locust muscle QUIS-R and that this anaesthetic dissociates only slowly from its blocking site(s). |
| Databáze: | OpenAIRE |
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