The Varicella-zoster virus ORF54 gene product encodes the capsid portal protein, pORF54
| Autor: | Denise M. Burnside, Debra M. Sherman, Robert J. Visalli, Melissa A. Visalli, Alexander J. Howard |
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| Rok vydání: | 2012 |
| Předmět: |
Herpesvirus 3
Human Cancer Research viruses Guinea Pigs Molecular Sequence Data Biology medicine.disease_cause Herpes Zoster Article Virus Cell Line Gene product Open Reading Frames Virology medicine Animals Humans Gene Antiserum Varicella zoster virus virus diseases Fusion protein Molecular biology Open reading frame Infectious Diseases Capsid Capsid Proteins |
| Zdroj: | Virus Research. 167:102-105 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2012.03.013 |
| Popis: | The Varicella-zoster virus (VZV) ORF54 gene was characterized using a guinea pig antiserum prepared to a GST-pORF54 fusion protein. A protein of the predicted size, 87kDa, was detected in VZV-infected MeWo cells but not in mock-infected cells. Sucrose density gradient fractionation of pORF54 expressed in a recombinant baculovirus system resulted in samples containing enriched amounts of pORF54. Electron microscopic analysis suggested that the ORF54 gene encodes a protein that assembles into ring-like portal structures similar to those observed for numerous bacteriophages and other herpesviruses. |
| Databáze: | OpenAIRE |
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