Identifying and exploring biohydrogenating rumen bacteria with emphasis on pathways including trans-10 intermediates

Autor: Jeyamalar Jeyanathan, Lore Dewanckele, Bruno Vlaeminck, Veerle Fievez
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Microbiology (medical)
Rumen
Conjugated linoleic acid
Biohydrogenation
lcsh:QR1-502
Biology
Microbiology
lcsh:Microbiology
Gas Chromatography-Mass Spectrometry
COMMUNITY COMPOSITION
03 medical and health sciences
Propionibacterium acnes
chemistry.chemical_compound
LIPID-METABOLISM
In vivo
Pure cultures
Animals
SUBACUTE RUMINAL ACIDOSIS
Biomass
030304 developmental biology
DAIRY-COWS
Bacteriological Techniques
0303 health sciences
Inoculation
0402 animal and dairy science
Biology and Life Sciences
Lipid metabolism
MILK-FAT
04 agricultural and veterinary sciences
CONJUGATED LINOLEIC-ACID
Hydrogen-Ion Concentration
Trans Fatty Acids
trans-11 totrans-10 shift
biology.organism_classification
040201 dairy & animal science
OIL
PRODUCTS
trans-11 to trans-10 shift
chemistry
Cuitbacterium acnes
NUTRITIONAL REGULATION
UNSATURATED FATTY-ACIDS
Bacteria
Research Article
Butyrivibrio fibrisolvens
Zdroj: BMC MICROBIOLOGY
BMC Microbiology
BMC Microbiology, Vol 20, Iss 1, Pp 1-21 (2020)
ISSN: 1471-2180
Popis: Background Bacteria involved in ruminal formation of trans-10 intermediates are unclear. Therefore, this study aimed at identifying rumen bacteria that produce trans-10 intermediates from 18-carbon unsaturated fatty acids. Results Pure cultures of 28 rumen bacterial species were incubated individually in the presence of 40 μg/mL 18:3n-3, 18:2n-6 or trans-11 18:1 under control or lactate-enriched (200 mM Na lactate) conditions for 24 h. Of the 28 strains, Cutibacterium acnes (formerly Propionibacterium acnes) was the only bacterium found to produce trans-10 intermediates from 18:3n-3 and 18:2n-6, irrespective of the growth condition. To further assess the potential importance of this species in the trans-11 to trans-10 shift, different biomass ratios of Butyrivibrio fibrisolvens (as a trans-11 producer) and C. acnes were incubated in different growth media (control, low pH and 22:6n-3 enriched media) containing 40 μg/mL 18:2n-6. Under control conditions, a trans-10 shift, defined in the current study as trans-10/trans-11 ≥ 0.9, occurred when the biomass of C. acnes represented between 90 and 98% of the inoculum. A low pH or addition of 22:6n-3 inhibited cis-9, trans-11 CLA and trans-10, cis-12 CLA formation by B. fibrisolvens and C. acnes, respectively, whereby C. acnes seemed to be more tolerant. This resulted in a decreased biomass of C. acnes required at inoculation to induce a trans-10 shift to 50% (low pH) and 90% (22:6n-3 addition). Conclusions Among the bacterial species studied, C. acnes was the only bacterium that have the metabolic ability to produce trans-10 intermediates from 18:3n-3 and 18:2n-6. Nevertheless, this experiment revealed that it is unlikely that C. acnes is the only or predominant species involved in the trans-11 to trans-10 shift in vivo.
Databáze: OpenAIRE
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