Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields
| Autor: | John Peter Rickgauer, David W. Tank, Karl Deisseroth |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Neurons Fluorescence-lifetime imaging microscopy General Neuroscience Place cell Mice Transgenic Optogenetics Biology Hippocampal formation Hippocampus Article Photostimulation Mice Inbred C57BL Functional imaging Mice medicine.anatomical_structure Space Perception medicine Animals Premovement neuronal activity Neuron Neuroscience Photic Stimulation |
| Zdroj: | Nature Neuroscience. 17:1816-1824 |
| ISSN: | 1546-1726 1097-6256 |
| DOI: | 10.1038/nn.3866 |
| Popis: | Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron’s coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, λ = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, λ = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or ‘biasing’, to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields. |
| Databáze: | OpenAIRE |
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