Isolation and identification of leukocyte populations in intracranial blood collected during mechanical thrombectomy
| Autor: | Benjamin C Shaw, Ann M. Stowe, Melissa Kesler, Jadwiga Turchan-Cholewo, Thomas A. Ujas, Chintan Rupareliya, G Benton Maglinger, Keith R. Pennypacker, Justin F. Fraser, Mathias Gelderblom, Stephen Grupke |
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| Rok vydání: | 2021 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine medicine.medical_specialty Isolation (health care) emergent large vessel occlusion (ELVO) T cells 03 medical and health sciences 0302 clinical medicine Internal medicine ischemic stroke medicine Humans cardiovascular diseases Registries Aged Thrombectomy Aged 80 and over business.industry flow cytometry Original Articles Middle Aged Cerebrovascular endothelium Stroke Mechanical thrombectomy Carotid Arteries 030104 developmental biology Neurology Ischemic stroke Cardiology Arterial blood Female Neurology (clinical) Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism |
| ISSN: | 1559-7016 0271-678X |
| DOI: | 10.1177/0271678x211028496 |
| Popis: | Using standard techniques during mechanical thrombectomy, the Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC) protocol (NCT03153683) isolates intracranial arterial blood distal to the thrombus and proximal systemic blood in the carotid artery. We augmented the current protocol to study leukocyte subpopulations both distal and proximal to the thrombus during human stroke (n = 16 patients), and from patients with cerebrovascular disease (CVD) undergoing angiography for unrelated conditions (e.g. carotid artery stenosis; n = 12 patients). We isolated leukocytes for flow cytometry from small volume (+ T cells and classical dendritic cells were significantly lower than CVD controls and correlated to within-patient edema volume and last known normal. This novel protocol successfully isolates leukocytes from small volume intracranial blood samples of stroke patients at time of mechanical thrombectomy and can be used to confirm preclinical results, as well as identify novel targets for immunotherapies. |
| Databáze: | OpenAIRE |
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