Analysis of Lung Flute–collected Sputum for Lung Cancer Diagnosis
| Autor: | Maria A. Guarnera, Feng Jiang, Jian Su, Sanford A. Stass, Nigar Anjuman, Howard Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Sputum Cytology Pathology Lung flute diagnosis Expectorate Gastroenterology lung tumor Lung Flute 03 medical and health sciences 0302 clinical medicine fluids and secretions Internal medicine medicine Sampling (medicine) Respiratory system Lung cancer genes Original Research Pharmacology lcsh:R5-920 business.industry Biochemistry (medical) Cancer sputum respiratory system medicine.disease 3. Good health respiratory tract diseases 030228 respiratory system 030220 oncology & carcinogenesis Molecular Medicine Sputum medicine.symptom business lcsh:Medicine (General) |
| Zdroj: | Biomarker Insights, Vol 2015, Iss 10, Pp 55-61 (2015) Biomarker Insights Biomarker Insights, Vol 10 (2015) |
| ISSN: | 1177-2719 |
| Popis: | Molecular analysis of sputum can help diagnose lung cancer. We have demonstrated that Lung Flute can be used to collect sputum from individuals who cannot spontaneously expectorate sputum. The objective of this study is to further evaluate the performance of the Lung Flute by comparing the characteristics of parallel samples collected with and without the Lung Flute and the usefulness for diagnosis of lung cancer. Fifty-six early-stage lung cancer patients (40 current smokers and 16 former smokers) and 73 cancer-free individuals (52 current smokers and 21 former smokers) were instructed to spontaneously cough and use Lung Flute for sputum sampling. Sputum cytology and polymerase chain reaction analysis of three miRNAs (miRs-21, 31, and 210) were performed in the specimens. All 92 current smokers and 11 (28.7%) of 37 former smokers spontaneously expectorated sputum and also produced sputum when using the Lung Flute. Twenty-seven former smokers (70.3%) who could not spontaneously expectorate sputum, however, were able to produce sputum when using the Lung Flute. The specimens were of low respiratory origin without contamination from other sources, eg, saliva. There was no difference of sputum volume and cell populations, diagnostic efficiency of cytology, and analysis of the miRNAs in the specimens collected by the two approaches. Analysis of the sputum miRNAs produced 83.93% sensitivity and 87.67% specificity for identifying lung cancer. Therefore, sputum collected by the Lung Flute has comparable features as spontaneously expectorated sputum. Using the Lung Flute enables former smokers who cannot spontaneously expectorate to provide adequate sputum to improve sputum collection for lung cancer diagnosis. |
| Databáze: | OpenAIRE |
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