Performance of a highly sensitive rapid diagnostic test (HS-RDT) for detecting malaria in peripheral and placental blood samples from pregnant women in Colombia
| Autor: | Iveth J. González, Ana Campillo, Ana María Vásquez, Ana Catalina Medina, Alberto Tobón-Castaño, Xavier C. Ding, Gabriel Jaime Vélez, Maritza Posada |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Plasmodium
Embryology Physiology Maternal Health Placenta lcsh:Medicine Artificial Gene Amplification and Extension Polymerase Chain Reaction 0302 clinical medicine Pregnancy Prenatal Diagnosis Medicine and Health Sciences 030212 general & internal medicine Pregnancy Complications Infectious lcsh:Science health care economics and organizations Protozoans Rapid diagnostic test Hematologic Tests Multidisciplinary biology Obstetrics Malarial Parasites Eukaryota Body Fluids Blood medicine.anatomical_structure Female Anatomy medicine.symptom Research Article Adult medicine.medical_specialty 030231 tropical medicine Colombia Research and Analysis Methods Sensitivity and Specificity Asymptomatic Young Adult 03 medical and health sciences Antenatal Care Parasite Groups parasitic diseases Parasitic Diseases medicine Humans Molecular Biology Techniques Molecular Biology Retrospective Studies Diagnostic Tests Routine business.industry lcsh:R Organisms Reproductive System Biology and Life Sciences Retrospective cohort study Plasmodium falciparum Tropical Diseases equipment and supplies medicine.disease biology.organism_classification Parasitic Protozoans Malaria Women's Health lcsh:Q Parasitology business Apicomplexa Nested polymerase chain reaction Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 8, p e0201769 (2018) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0201769 |
| Popis: | Background Pregnancy poses specific challenges for the diagnosis of Plasmodium falciparum infection due to parasite sequestration in the placenta, which translates in low circulation levels in peripheral blood. The aim of this study is to assess the performance of a new highly sensitive rapid diagnostic test (HS-RDT) for the detection of malaria in peripheral and placental blood samples from pregnant women in Colombia. Methods This is a retrospective study using 737 peripheral and placental specimens collected from pregnant women in Colombian malaria-endemic regions. Light microscopy (LM), conventional rapid diagnostic tests (Pf/Pv RDT and Pf RDT), and HS-RDT testing were performed. Diagnostic accuracy endpoints of LM, HS-RDT and RDTs were compared with nested polymerase chain reaction (nPCR) as the reference test. Results In comparison with nPCR, the sensitivity of HS-RDT, Pf RDT, Pf/Pv RDT and LM to detect infection in peripheral samples was 85.7% (95% CI = 70.6–93.7), 82.8% (95% CI = 67.3–91.9), 77.1% (95% CI = 61.0–87.9) and 77.1% (95% CI = 61.0–87.9) respectively. The sensitivity to detect malaria in asymptomatic women, was higher with HS-RDT, where LM and Pf/Pv RDT missed half of infections detected by nPCR, but differences were not significant. Overall, specificity was similar for all tests (>99.0%). In placental blood, the prevalence of infection by P. falciparum by nPCR was 2.8% (8/286), by HS-RDT was 1% and by conventional RDTs (Pf RDT and Pf/Pv RDT) and LM was 0.7%. The HS-RDT detected placental infections in peripheral blood that were negative by LM and Pf/Pv RDT, however the number of positive placentas was low. Conclusions The sensitivity of HS-RDT to detect P. falciparum infections in peripheral and placental samples from pregnant women was slightly better compared to routinely used tests during ANC visits and at delivery. Although further studies are needed to guide recommendations on the use of the HS-RDT for malaria case management in pregnancy, this study shows the potential value of this test to diagnose malaria in pregnancy in low-transmission settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|