Cissampelos sympodialis has anti-viral effect inhibiting dengue non-structural viral protein-1 and pro-inflammatory mediators
| Autor: | Ana Luisa de Araujo Lima, Claire Fernandes Kubelka, Marcia Regina Piuvezam, Fagner Carvalho Leite, Cintia Ferreira Marinho, Luciana Gomes Fialho, Cíntia da Silva Mello, José Maria Barbosa Filho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cissampelos sympodialis Viral protein viruses Cell NS1 lcsh:RS1-441 Pharmacology Dengue virus medicine.disease_cause Dengue fever lcsh:Pharmacy and materia medica Dengue Pharmacology Toxicology and Pharmaceutics(all) 03 medical and health sciences medicine General Pharmacology Toxicology and Pharmaceutics Menispermaceae Warifteine Cissampelos Huh-7 cells biology biology.organism_classification medicine.disease In vitro 030104 developmental biology medicine.anatomical_structure Hepatocyte Immunology Cytokines |
| Zdroj: | Revista Brasileira de Farmacognosia, Volume: 26, Issue: 4, Pages: 502-506, Published: AUG 2016 Revista Brasileira de Farmacognosia v.26 n.4 2016 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA Revista Brasileira de Farmacognosia, Vol 26, Iss 4, Pp 502-506 Revista Brasileira de Farmacognosia, Vol 26, Iss 4, Pp 502-506 (2016) |
| Popis: | Dengue is the most important viral infection transmitted among humans by arthropod-borne. There are currently no vaccines or specific therapeutical treatment. Therefore, immunomodulatory compounds from plants have been widely examined for their antiviral effects. Cissampelos sympodialis Eichler, Menispermaceae, has scientifically proven to present immunomodulatory activities. Here we assessed the antiviral activity of leaf hydroalcoholic extract, warifteine or methylwarifteine from C. sympodialis in an in vitro dengue virus infection model. The results demonstrated that leaf hydroalcoholic extract or warifteine/methylwarifteine treatment did not reduce dengue virus-Ag+ hepatocyte (Huh-7 cell) rates in present experimental conditions. However, we assessed the potential antiviral effect of leaf hydroalcoholic extract or warifteine/methylwarifteine on dengue virus-infection by the production of inflammatory molecules, TNF-α, MIF, IL-8 and PGE2. Dengue virus infection enhanced TNF-α, MIF, IL-8 and PGE2 production in infected Huh-7 cells and leaf hydroalcoholic extract but not warifteine/methylwarifteine treatments, significantly reduced these molecules in infected cells. In dengue virus-infected Huh-7 cells, non-structural protein-1 is produced and leaf hydroalcoholic extract significantly inhibited it independently of alkaloids. Our findings imply that leaf hydroalcoholic extract may attenuate dengue virus infection in Huh-7 cells by inhibiting the enhanced of pro-inflammatory mediators and non-structural protein-1 production induce by dengue virus independently of warifteine/methywarifteine its major compound. Keywords: Cissampelos sympodialis, Dengue, Huh-7 cells, Cytokines, NS1, Warifteine |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|