Vaccination trials of sea bass, Dicentrarchus labrax (L.), against Photobacterium damsela subsp. piscicida, using novel vaccine mixtures
| Autor: | V Bakopoulos, Alexandra Adams, L Gusmani, Marco Galeotti, G J Dimitriadis, Donatella Volpatti |
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| Rok vydání: | 2003 |
| Předmět: |
Veterinary (miscellaneous)
Administration Oral Aquaculture Aquatic Science Microbiology Fish Diseases medicine Animals Sea bass Pathogen Photobacterium damsela biology business.industry Photobacterium Vaccination medicine.disease biology.organism_classification Bacterial vaccine Bacterial Vaccines Dicentrarchus Bass business Gram-Negative Bacterial Infections Pasteurellosis Injections Intraperitoneal |
| Zdroj: | Journal of fish diseases. 26(2) |
| ISSN: | 0140-7775 |
| Popis: | Bacterial cells of the marine fish pathogen Photobacterium damsela subsp. piscicida were grown in novel culture media. A mixture of whole cells and extracellular components was inactivated and used in bath, intraperitoneal (i.p.) and oral vaccination of sea bass, Dicentrarchus labrax, employing two sizes of fish. A commercial vaccine was used for comparative purposes. Control and immunized fish were either bath or intraperitoneally challenged 6 and 12 weeks post-vaccination. Small fish had significantly higher relative percentage survival with the novel vaccine mixture both at 6 and 12 weeks post-vaccination by bath, in comparison with the commercial vaccine. No protection was afforded at 6 or 12 weeks post-immunization by either vaccine after challenge via i.p. injection. Sea bass (1.5-2 g) intraperitoneally vaccinated with various adjuvanted vaccine mixtures were not protected against pasteurellosis. In contrast, larger sea bass (20 g) benefited from vaccination with the novel vaccine mixtures. Intraperitoneal challenge with the pathogen resulted in protection in both fish groups vaccinated with novel vaccine mixtures, whereas control fish suffered high mortalities (> 80%). Orally vaccinated fish were immersion challenged with the pathogen. At 6 and 12 weeks post-vaccination the control fish had a high mortality and the fish vaccinated with the novel vaccine mixture achieved good protection. |
| Databáze: | OpenAIRE |
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