Intravenous Arginine Administration Promotes Proangiogenic Cells Mobilization and Attenuates Lung Injury in Mice with Polymicrobial Sepsis
| Autor: | Chiu Li Yeh, Man Hui Pai, Yao Ming Shih, Sung Ling Yeh, Juey Ming Shih |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male proangiogenic cells Arginine Neovascularization Physiologic lcsh:TX341-641 Inflammation arginine Pharmacology Lung injury Article Proinflammatory cytokine Sepsis Angiopoietin sepsis 03 medical and health sciences Mice Angpt/Tie-2 lung injury medicine Animals Endothelial Progenitor Cells Nutrition and Dietetics Lung business.industry Bacterial Infections Lung Injury medicine.disease Receptor TIE-2 Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Immunology Injections Intravenous medicine.symptom business lcsh:Nutrition. Foods and food supply Angiopoietins Homeostasis Food Science |
| Zdroj: | Nutrients Nutrients, Vol 9, Iss 5, p 507 (2017) Nutrients; Volume 9; Issue 5; Pages: 507 |
| ISSN: | 2072-6643 |
| Popis: | This study investigated the influence of intravenous arginine (Arg) administration on alteration of circulating proangiogenic cells and remote lung injury in a model of polymicrobial sepsis. Mice were assigned to one normal control group (NC) and two sepsis groups that were induced by cecal ligation and puncture (CLP). One of the sepsis groups was injected with saline (SS), whereas the other (SA) was administered with a single bolus of 300 mg Arg/kg body weight via the tail vein 1 h after CLP. Septic mice were sacrificed at either 24 or 48 h after CLP, with their blood and lung tissues collected for analysis. Results showed that septic groups had higher proangiogenic cells releasing factors and proangiogenic cells percentage in blood. Also, concentration of inflammatory cytokines and expression of angiopoietin (Angpt)/Tie-2 genes in lung tissues were upregulated. Arg administration promoted mobilization of circulating proangiogenic cells while it downregulated the production of inflammatory cytokines and expression of Angpt/Tie-2 genes in the lung. The results of this investigation suggested that intravenous administration of Arg shortly after the onset of sepsis enhanced the mobilization of circulating proangiogenic cells, maintained the homeostasis of the Angpt/Tie-2 axis, and attenuated remote organ injury in polymicrobial sepsis. |
| Databáze: | OpenAIRE |
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