Electron beam triggered, free radical polymerization-derived monolithic capillary columns for high-performance liquid chromatography
| Autor: | Lutz Rosenkranz, Michael R. Buchmeiser, Bettina Schlemmer, Rajendar Bandari |
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| Rok vydání: | 2008 |
| Předmět: |
Monolithic HPLC column
Free Radicals Scanning electron microscope Capillary action Polymers Radical polymerization Analytical chemistry Electrons Methacrylate Biochemistry High-performance liquid chromatography Analytical Chemistry 2-Propanol chemistry.chemical_compound Derivatization Chromatography High Pressure Liquid Reproducibility Chromatography Organic Chemistry Proteins Reproducibility of Results General Medicine chemistry Acrylates Dodecanol Methacrylates |
| Zdroj: | Journal of chromatography. A. 1216(13) |
| ISSN: | 1873-3778 |
| Popis: | Monolithic capillary columns were prepared via electron beam triggered free radical polymerization within the confines of 0.2 and 0.1 mm I.D. capillary columns using ethyl methacrylate and trimethylolpropane triacrylate as monomers as well as 2-propanol, 1-dodecanol and toluene as porogenic system. The influence of column diameter on reproducibility and separation performance was investigated. For evaluation, a protein standard consisting of five proteins in the range of 5800–66 000 g mol −1 was used. Reproducibility was checked by determining the relative standard deviations in retention times, peak widths at half height, asymmetry and resolution. Excellent run-to-run reproducibility was found for both 0.2 and 0.1 mm I.D. columns; batch-to-batch reproducibility was good for both column types. In order to enhance the non-polar character of the monolithic columns, lauryl methacrylate-based capillary columns were prepared. These were successfully used for the separation of proteins and a cytochrome c digest. |
| Databáze: | OpenAIRE |
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