The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress
| Autor: | Michael Melzer, Andrea Matros, Katja Witzel, Tariq Aftab, Uwe Bertsch, Hans-Peter Mock, Eswarayya Ramireddy, Gotthard Kunze, Twan Rutten |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
abiotic stress
QH301-705.5 Saccharomyces cerevisiae Mutant seedling Plant Roots Salt Stress Article Catalysis salinity Inorganic Chemistry Gene Expression Regulation Plant Stress Physiological Lectins Complementary DNA Arabidopsis thaliana Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Plant Proteins Hordeum vulgare biology Abiotic stress cDNA library Organic Chemistry food and beverages Hordeum Salt Tolerance General Medicine biology.organism_classification apoplast Adaptation Physiological functional screening Computer Science Applications Chemistry Phenotype Biochemistry Seedlings Jacalin Plant Lectins |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 10248, p 10248 (2021) International Journal of Molecular Sciences Volume 22 Issue 19 International journal of molecular sciences, 22(19):10248 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex for the transfection of a salt-stress-sensitive yeast strain (Saccharomyces cerevisiae YSH818 Δhog1 mutant). From the retrieved cDNA sequences conferring salt tolerance to the yeast mutant, eleven contained the coding sequence of a jacalin-related lectin (JRL) that shows homology to the previously identified JRL horcolin from barley coleoptiles that we therefore named the gene HvHorcH. The detection of HvHorcH protein in root extracellular fluid suggests a secretion under stress conditions. Furthermore, HvHorcH exhibited specificity towards mannose. Protein abundance of HvHorcH in roots of salt-sensitive or salt-tolerant barley cultivars were not trait-specific to salinity treatment, but protein levels increased in response to the treatment, particularly in the root tip. Expression of HvHorcH in Arabidopsis thaliana root tips increased salt tolerance. Hence, we conclude that this protein is involved in the adaptation of plants to salinity. |
| Databáze: | OpenAIRE |
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