Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis
| Autor: | Juan Olate, Juan Pablo Henríquez, Jaime Fuentealba, R. Maldonado-Agurto, G. Toro, Marcela Torrejón, Tania Campos, Miguel Albistur, María Victoria Hinrichs, Cecilia Arriagada |
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| Rok vydání: | 2011 |
| Předmět: |
Neural Tube
Xenopus Molecular Sequence Data Embryonic Development Xenopus Proteins Synaptic Transmission hemic and lymphatic diseases Genetics medicine Asymmetric cell division Animals Guanine Nucleotide Exchange Factors Tissue Distribution Amino Acid Sequence Cloning Molecular Molecular Biology Gene biology Embryogenesis Asymmetric Cell Division Neural tube Neural crest Brain Gene Expression Regulation Developmental biology.organism_classification medicine.anatomical_structure Signal transduction Neural development Developmental Biology Signal Transduction |
| Zdroj: | GENE EXPRESSION PATTERNS Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1872-7298 |
| Popis: | RIC-8 is a highly conserved protein that promotes G protein signaling as it acts as a Guanine nucleotide Exchanging Factor (GEF) over a subset of Gα subunits. In invertebrates, RIC-8 plays crucial roles in synaptic transmission as well as in asymmetric cell division. As a first step to address further studies on RIC-8 function in vertebrates, here we have cloned a ric-8 gene from Xenopus tropicalis (xtric-8) and determined its spatiotemporal expression pattern throughout embryogenesis. The xtric-8 transcript is expressed maternally and zygotically and, as development proceeds, it shows a dynamic expression pattern. At early developmental stages, xtric-8 is expressed in the animal hemisphere, whereas its expression is later restricted to neural tissues, such as the neural tube and the brain, as well as in the eye and neural crest-derived structures, including those of the craniofacial region. Together, our findings suggest that RIC-8 functions are related to the development of the nervous system. |
| Databáze: | OpenAIRE |
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