ABCA1 contributes to macrophage deposition of extracellular cholesterol
| Autor: | Ying Liu, Lia Addadi, Janet Chang, Alan T. Remaley, Sebastian R. Freeman, Howard S. Kruth, Neta Varsano, Xueting Jin, Boris L. Vaisman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Hydrocarbons
Fluorinated Lipoproteins Probucol QD415-436 Biochemistry Extracellular matrix chemistry.chemical_compound Mice Endocrinology medicine Extracellular Macrophage Animals Humans Liver X receptor Research Articles ATP Binding Cassette Transporter Subfamily G Member 1 Liver X Receptors Sulfonamides biology Apolipoprotein A-I Cholesterol Macrophages Reverse cholesterol transport Cell Biology Atherosclerosis Orphan Nuclear Receptors Cell biology Extracellular Matrix chemistry high density lipoprotein ABCA1 biology.protein lipids (amino acids peptides and proteins) ATP-Binding Cassette Transporters ATP binding cassette transporter A1 TO901317 Lipoproteins HDL medicine.drug ATP Binding Cassette Transporter 1 |
| Zdroj: | Journal of Lipid Research, Vol 56, Iss 9, Pp 1720-1726 (2015) |
| Popis: | We previously reported that cholesterol-enriched macrophages excrete cholesterol into the extracellular matrix. A monoclonal antibody that detects cholesterol microdomains labels the deposited extracellular particles. Macro-phage deposition of extracellular cholesterol depends, in part, on ABCG1, and this cholesterol can be mobilized by HDL components of the reverse cholesterol transport process. The objective of the current study was to determine whether ABCA1 also contributes to macrophage deposition of extracellular cholesterol. ABCA1 functioned in extracellular cholesterol deposition. The liver X receptor agonist, TO901317 (TO9), an ABCA1-inducing factor, restored cholesterol deposition that was absent in cholesterol-enriched ABCG1(-/-) mouse macrophages. In addition, the ABCA1 inhibitor, probucol, blocked the increment in cholesterol deposited by TO9-treated wild-type macrophages, and completely inhibited deposition from TO9-treated ABCG1(-/-) macrophages. Lastly, ABCA1(-/-) macrophages deposited much less extracellular cholesterol than wild-type macrophages. These findings demonstrate a novel function of ABCA1 in contributing to macrophage export of cholesterol into the extracellular matrix. |
| Databáze: | OpenAIRE |
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