Regulation of host-infection ability in the grass-symbiotic fungus Epichloë festucae by histone H3K9 and H3K36 methyltransferases
| Autor: | Nazanin Noorifar, Kimberly A. Green, Murray P. Cox, Tracy K. Hale, Arvina Ram, David J. Winter, Barry Scott, Yonathan Lukito, Linda J. Johnson, Tetsuya Chujo, Kate Lee |
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| Rok vydání: | 2020 |
| Předmět: |
Genetics
0303 health sciences Methyltransferase 030306 microbiology Epichloe Mutant Methyltransferases Biology Poaceae Microbiology Phenotype Transcriptome Fungal Proteins Histones 03 medical and health sciences Histone Histone methyltransferase Gene Expression Regulation Fungal Histone methylation biology.protein Symbiosis Gene Ecology Evolution Behavior and Systematics 030304 developmental biology |
| Zdroj: | Environmental microbiologyReferences. 23(4) |
| ISSN: | 1462-2920 |
| Popis: | Recent studies have identified key genes that control the symbiotic interaction between Epichloe festucae and Lolium perenne. Here we report on the identification of specific E. festucae genes that control host infection. Deletion of setB, which encodes a homolog of the H3K36 histone methyltransferase Set2/KMT3, reduced histone H3K36 trimethylation and led to severe defects in colony growth and hyphal development. The E. festucae ΔclrD mutant, which lacks the gene encoding the homolog of the H3K9 methyltransferase KMT1, displays similar developmental defects. Both mutants are completely defective in their ability to infect L. perenne. Alleles that complement the culture and plant phenotypes of both mutants also complement the histone methylation defects. Co-inoculation of either ΔsetB or ΔclrD with the wild-type strain enables these mutants to colonize the host. However, successful colonization by the mutants resulted in death or stunting of the host plant. Transcriptome analysis at the early infection stage identified four fungal candidate genes, three of which encode small-secreted proteins, that are differentially regulated in these mutants compared to wild-type. Deletion of crbA, which encodes a putative carbohydrate binding protein, resulted in significantly reduced host infection rates by E. festucae. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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