Release of tumor necrosis factor-alpha from bovine alveolar macrophages stimulated with bovine respiratory viruses and bacterial endotoxins
| Autor: | G.K. Allen, J.N. Berg, S.E. Bienhoff |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
animal diseases viruses Immunology Blotting Western Cattle Diseases Monoclonal antibody medicine.disease_cause Virus Herpesviridae Microbiology Macrophages Alveolar medicine Escherichia coli Animals Pasteurella multocida Respiratory Tract Infections Cells Cultured Herpesvirus 1 Bovine General Veterinary biology Tumor Necrosis Factor-alpha Antibodies Monoclonal Macrophage Activation biology.organism_classification Bovine herpesvirus 1 Parainfluenza Virus 3 Human Respiratory Syncytial Viruses Endotoxins medicine.anatomical_structure biology.protein Tumor necrosis factor alpha Cattle Pasteurella Pulmonary alveolus Antibody |
| Zdroj: | Veterinary immunology and immunopathology. 30(4) |
| ISSN: | 0165-2427 |
| Popis: | The release of tumor necrosis factor-alpha (TNF-alpha) from cultured bovine alveolar macrophages (BAM) was evaluated following stimulation of BAM with bovine herpesvirus-1 (BHV-1), parainfluenza-3 (PI-3) virus, bovine respiratory syncytial virus (BRSV), Escherichia coli 0111:B4 endotoxin, Pasteurella haemolytica type 1 endotoxin, Pasteurella multocida endotoxin, and virus/endotoxin combinations. A cytotoxic assay system using Georgia bovine kidney cells as targets was used to measure TNF-alpha activity. The cytotoxic activity was neutralized by an anti-human TNF-alpha monoclonal antibody. Stimulation of BAM with 1 median tissue culture infectious dose (TCID50) of live or ultraviolet (UV)-inactivated PI-3 virus/cell resulted in release of TNF-alpha in significantly (P less than 0.05) higher amounts than sham-induced BAM. The quantities of TNF-alpha released after live or UV-inactivated BHV-1 or BRSV induction were not significantly higher than sham-induced BAM. E. coli 0111:B4, P. haemolytica type 1 and P. multocida endotoxins stimulated TNF-alpha release in a dose-dependent manner. Sequential exposure of BAM to 1 TCID50 per cell of either live BHV-1, PI-3 virus or BRSV and then 5 micrograms ml-1 of either E. coli 0111:B4, P. haemolytica type 1 or P. multocida endotoxin caused a significant (P less than 0.05) reduction in detectable TNF-alpha in seven of nine virus/endotoxin combinations tested, when compared with 5 micrograms ml-1 of endotoxin alone. Parainfluenza-3 virus/endotoxin combinations stimulated higher TNF-alpha release when compared with other virus/endotoxin combinations. Five out of six test animals had serum-neutralizing antibodies to PI-3 virus, one out of six had serum-neutralizing antibodies to BHV-1, and two out of six had serum-neutralizing antibodies to BRSV, suggesting a possible relationship between serum neutralizing antibodies and TNF-alpha release from in vitro cultivated BAM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|