Gamma-aminobutyric acid (GABA) immunoreactivity in the mouse adrenal gland
| Autor: | Hiromichi Iuchi, Yohichi Satoh, Katsushi Ishikawa, Yukio Oomori, Hiroshi Tanaka, Kazuhiro Nakaya, Kazuyuki Ono |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pathology Histology Immunocytochemistry Biology gamma-Aminobutyric acid Mice Norepinephrine Nerve Fibers Internal medicine medicine Animals Chromaffin Granules Microscopy Immunoelectron Molecular Biology gamma-Aminobutyric Acid Medulla Mice Inbred BALB C Adrenal gland Adrenal cortex Cell Biology General Medicine Immunohistochemistry Ganglion Medical Laboratory Technology medicine.anatomical_structure Endocrinology Microscopy Fluorescence nervous system Axoplasm Adrenal Medulla Anatomy General Agricultural and Biological Sciences Adrenal medulla medicine.drug |
| Zdroj: | Histochemistry. 100:203-213 |
| ISSN: | 1432-119X 0301-5564 |
| Popis: | Gamma-aminobutyric acid (GABA) immunoreactivity was revealed by immunocytochemistry in the mouse adrenal gland at the light and electron microscopic levels. Groups of weakly or faintly GABA immunoreactive chromaffin cells were often seen in the adrenal medulla. By means of immunohistochemistry combined with fluorescent microscopy, these GABA immunoreactive chromaffin cells showed noradrenaline fluorescence. The immunoreaction product was seen mainly in the granular cores of these noradrenaline cells. These results suggest the co-existence of GABA and noradrenaline within the chromaffin granules. Sometimes thick or thin bundles of GABA immunoreactive nerve fibers with or without varicosities were found running through the cortex directly into the medulla. In the medulla, GABA immunoreactive varicose nerve fibers were numerous and were often in close contact with small adrenaline cells and large ganglion cells; a few, however, surrounded clusters of the noradrenaline cells, where membrane specializations were formed. Single GABA immunoreactive nerve fibers, and thin or thick bundles of the immunoreactive varicose nerve fibers ran along the blood vessels in the medulla. The immunoreaction deposits were observed diffusely in the axoplasm and in small agranular vesicles of the GABA immunoreactive nerve fibers. Since no ganglion cells with GABA immunoreactivity were found in the adrenal gland, the GABA immunoreactive nerve fibers are regarded as extrinsic in origin. |
| Databáze: | OpenAIRE |
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