Microarray expression profile analysis of aberrant long non-coding RNAs in esophageal squamous cell carcinoma
| Autor: | Mei Lin, Hong Yu, Guo-Jun Zhao, Peng-Cheng Wang, Juan Yao, Jing Wu, Zheng-dong Wu, Ping Chen, Jun-Xing Huang, Jun Ye |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cancer Research Microarray Esophageal Neoplasms Cellular differentiation Biology medicine.disease_cause Bioinformatics 03 medical and health sciences medicine Humans Gene Regulatory Networks RNA Messenger Neoplasm Metastasis neoplasms Oligonucleotide Array Sequence Analysis Regulation of gene expression Oncogene Gene Expression Profiling Cell cycle Survival Analysis digestive system diseases Gene expression profiling Gene Expression Regulation Neoplastic 030104 developmental biology Oncology Tumor progression Cancer research Carcinoma Squamous Cell Female RNA Long Noncoding Esophageal Squamous Cell Carcinoma Carcinogenesis |
| Zdroj: | International journal of oncology. 48(6) |
| ISSN: | 1791-2423 |
| Popis: | Increasing evidence indicates that long non-coding RNA (lncRNA) plays an important role in tumorigenesis. However, the function and regulatory mechanism of lncRNAs are still unclear in esophageal squamous cell carcinoma (ESCC). To address this challenge, we screened lncRNAs expression profiles in 3 pairs of ESCC and matched non-cancerous tissues by microarray assay and identified the relationship between lncRNAs expression in ESCC tissue and clinicopathological characteristics and prognosis of patients with ESCC. We found 182 lncRNAs that were significantly differently expressed in ESCC tissues versus the matched non-cancerous tissues. Gene ontology and pathway analysis results suggested that the primary biological processes of these genes were involved in extracellular matrix, immune responses, cell differentiation and cell proliferation. Through cis and trans analyzing, we found 4 lncRNAs (ENST00000480669, NONHSAT104436, NONHSAT126998 and NONHSAT112918) may play important roles in tumorigenesis of ESCC. The four lncRNAs were checked in 73 patients with ESCC. The results showed that they mainly related to tumor metastasis. Kaplan-Meier survival analysis showed that high expression of NONHSAT104436, NONHSAT126998 and low expression of ENST00000480669 were related to poor 3-year overall survival (P=0.003, 0.032 and 0.040, respectively). Multivariate analysis showed that NONHSAT104436 was an independent prognostic factor (P=0.017). Thus we concluded that, lncRNAs showed differently expression patterns in ESCC versus matched non-cancerous tissues, and aberrantly expressed lncRNA may play important roles in ESCC development and progression. Interestingly, the overexpression of NONHSAT104436 was tightly correlated with distant metastasis and, poor survival rate, which might indicate that NONHSAT104436 might play a very important part in ESCC tumor progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|