Interleukin 20 protein locates to distinct mononuclear cells in psoriatic skin
| Autor: | Kristian Otkjaer, Claus Johansen, Lars Iversen, John Rømer, Rikke Bech, Ralf Agger, Knud Kragballe, Thomas Vorup-Jensen, Svend Birkelund |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Keratinocytes
Langerin medicine.medical_treatment Interleukin-1beta Enzyme-Linked Immunosorbent Assay Dermatology Biochemistry Peripheral blood mononuclear cell Antigens CD1 Interleukin 20 Antigens CD medicine Humans Psoriasis Lectins C-Type Receptors Immunologic Molecular Biology Skin Membrane Glycoproteins Epidermis (botany) biology integumentary system Interleukins Keratin-14 Dendritic cell Molecular biology HEK293 Cells Mannose-Binding Lectins Dermal papillae medicine.anatomical_structure Cytokine CD4 Antigens Immunology Leukocytes Mononuclear biology.protein Epidermis Keratinocyte |
| Zdroj: | Bech, R, Otkjaer, K, Birkelund, S, Vorup-Jensen, T, Agger, R, Johansen, C, Iversen, L, Kragballe, K & Rømer, J 2014, ' Interleukin 20 protein locates to distinct mononuclear cells in psoriatic skin ', Experimental Dermatology, vol. 23, no. 5, pp. 349–351 . https://doi.org/10.1111/exd.12371 Bech, R, Otkjaer, K, Birkelund, S, Vorup-Jensen, T, Agger, R, Johansen, C, Iversen, L, Kragballe, K & Rømer, J 2014, ' Interleukin 20 protein locates to distinct mononuclear cells in psoriatic skin ', Experimental Dermatology Online . https://doi.org/10.1111/exd.12371 |
| DOI: | 10.1111/exd.12371 |
| Popis: | We previously demonstrated that mRNA for the pro-inflammatory cytokine interleukin 20 (IL-20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin. Here, we describe the distribution of IL-20 protein and the identity of the IL-20-positive cells in lesional psoriatic skin. We found that the main part of IL-20 immunoreactivity is present in mononuclear cells of the dermal papillae, and that the IL-20 positive cells located n the papillae were Langerin+, CD1a+, CD4+, and CD303+. These cells might be immature DC. In situ hybridization for IL-20 mRNA on non-lesional psoriatic skin, ex vivo stimulated with IL-1β revealed a co-localization between IL-20 mRNA and the keratinocyte marker CK14. No IL-20 mRNA was detected in the dermal mononuclear cells. Our results suggest that IL-20 is produced by keratinocytes, released into the epidermis, and then possibly taken up by papillary mononuclear cells. Our study supports that IL-20 is involved in the pathogenesis of psoriasis. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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