Increased Neuroligin 2 Levels in the Postsynaptic Membrane in Spinal Dorsal Horn may Contribute to Postoperative Pain
| Autor: | Ruijuan Guo, Danxu Ma, Huili Li, Ye Zhang, Xueyang Li, Yuqing Sun, Huihui Miao, Fangxiao Hong, Junfa Li, Yun Wang, Yun Guan, Ming Tian |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Spinal Cord Dorsal Horn medicine.medical_specialty Cell Adhesion Molecules Neuronal Synaptic Membranes Nerve Tissue Proteins AMPA receptor Rats Sprague-Dawley Synapse 03 medical and health sciences 0302 clinical medicine Western blot Downregulation and upregulation 030202 anesthesiology Postsynaptic potential Internal medicine medicine Animals Pain Postoperative medicine.diagnostic_test business.industry General Neuroscience Spinal cord Rats Endocrinology medicine.anatomical_structure Hyperalgesia medicine.symptom business Disks Large Homolog 4 Protein 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 382:14-22 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2018.04.028 |
| Popis: | Neuroligin 2 is a synaptic cell adhesion molecule that is mainly located in inhibitory synapses and is crucial in the regulation of synapse function through protein-protein interactions. However, researchers have not clearly determined whether neuroligin 2 is involved in the development of postoperative pain. In the current study, Western blot, immunofluorescence staining and co-immunoprecipitation were used to examine the critical role of neuroligin 2 in postoperative pain hypersensitivity. A small interfering ribonucleic acid (siRNA)-targeting neuroligin 2 was used to inhibit neuroligin 2 expression. Our data found that plantar incision induced postoperative pain hypersensitivity, which was characterized by paw withdrawal threshold and cumulative pain score. The upregulation of neuroligin 2 and GluR1 expression in the postsynaptic membranes of ipsilateral spinal dorsal horn was observed at 3 h and 1 day after plantar incision. Additionally, at 3 h after plantar incision, the amount of PSD-95 that was co-immunoprecipitated with neuroligin 2 antibody was significantly increased in the ipsilateral dorsal horn, as compared to that of the control group. Intrathecal pretreatment of siRNA-targeting neuroligin 2 to reduce the neuroligin 2 expression in the spinal cord significantly inhibited the pain hypersensitivity and reduced the synaptic targeting of GluR1 in ipsilateral dorsal horns. Our study indicates that the incision-induced interaction between neuroligin 2 and PSD-95 and subsequent synaptic targeting of GluR1 in ipsilateral dorsal horns contribute to postoperative pain hypersensitivity. |
| Databáze: | OpenAIRE |
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