MicroRNA-377 Counteracts With Cancer Stem Cell Phenotypes and Epithelial Mesenchymal Transformation by Targeting ZEB2 in Colon Cancer

Autor: Darebai Redati, Paerhati Shayimu, Aikeremu Yusufu, Aizimaiti Rehemutula, Rexida Jiapaer, Rousidan Tuerdi
Rok vydání: 2020
Předmět:
Male
Cancer Research
cancer stem cell
Epithelial-Mesenchymal Transition
Colorectal cancer
Biology
lcsh:RC254-282
miR-377
Metastasis
03 medical and health sciences
0302 clinical medicine
Cancer stem cell
Cell Line
Tumor
microRNA
medicine
Humans
metastasis
Epithelial–mesenchymal transition
Neoplasm Metastasis
030304 developmental biology
Aged
Cell Proliferation
Neoplasm Staging
Zinc Finger E-box Binding Homeobox 2
ZEB2
Aged
80 and over
0303 health sciences
Mesenchymal stem cell
EMT
Cell migration
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Gene Expression Regulation
Neoplastic
MicroRNAs
Oncology
colon cancer
030220 oncology & carcinogenesis
Colonic Neoplasms
Cancer research
Neoplastic Stem Cells
Female
RNA Interference
Original Article
Stem cell
Zdroj: Technology in Cancer Research & Treatment
Technology in Cancer Research & Treatment, Vol 19 (2020)
ISSN: 1533-0338
Popis: Dysregulated microRNAs (miRNAs) have been implicated in the pathogenic processes of colon cancer. Epithelial mesenchymal transition (EMT) promotes metastatic progression and cancer stem cells are closely involved in colon cancer proliferation and metastasis. Functional effects of miR-377 on colon cancer stem cell phenotypes and EMT were then determined in the present study. Firstly, reduced miR-377 was found in colon cancer tissues and cell lines. Results from flow cytometry, sphere formation and western blot assays showed that miR-377 knockdown increased number of ALDH+ cells and promoted sphere formation ability. Moreover, cell migration/invasion and EMT of colon cancer cells were suppressed by miR-377 over-expression. On the contrary, miR-377 mimics caused the reversed results. ZEB2 (zinc finger E box-binding homeobox 2) was then validated as a binding target of miR-377. ZEB2 over-expression reversed the inhibitory abilities of miR-377 on cancer stem cell phenotypes, EMT, migration and invasion. In conclusion, miR-377 regulates cancer stem cell phenotypes and EMT in colon cancer cells via regulation of ZEB2, suggesting a new therapeutic strategy for colon cancer treatment.
Databáze: OpenAIRE
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