The large GTPase dynamin2: a new player in connexin 43 gap junction endocytosis, recycling and degradation

Autor: Jim Dompierre, Dominique Segretain, Jerome Gilleron, Jean-Pierre Denizot, Céline Fiorini, Diane Carette, Georges Pointis, Eric Macia
Přispěvatelé: Centre méditérannéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de pharmacologie moléculaire et cellulaire (IPMC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Unité de neurosciences intégratives et computationnelles (UNIC), Centre National de la Recherche Scientifique (CNRS), Institut de Neurobiologie Alfred Fessard (INAF), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
Scaffold protein
Male
MESH: Cell Line
Tumor
media_common.quotation_subject
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Gap Junctions
Connexin
GTPase
Biology
Endocytosis
Transfection
Biochemistry
03 medical and health sciences
Dynamin II
0302 clinical medicine
MESH: Sertoli Cells
Cell Line
Tumor
Humans
Internalization
Cells
Cultured
030304 developmental biology
Dynamin
media_common
0303 health sciences
MESH: Humans
Sertoli Cells
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Transfection
Gap junction
Hydrazones
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Gap Junctions
Cell Biology
Receptor-mediated endocytosis
MESH: Connexin 43
MESH: Male
Cell biology
030220 oncology & carcinogenesis
Connexin 43
MESH: Dynamin II
MESH: Endocytosis
sense organs
biological phenomena
cell phenomena
and immunity
MESH: Hydrazones
MESH: Cells
Cultured
Zdroj: International Journal of Biochemistry and Cell Biology
International Journal of Biochemistry and Cell Biology, Elsevier, 2011, 43 (8), pp.1208-1217. ⟨10.1016/j.biocel.2011.04.014⟩
ISSN: 1878-5875
1357-2725
DOI: 10.1016/j.biocel.2011.04.014⟩
Popis: International audience; Connexins (Cx) are key regulators of cell proliferation, differentiation and apoptosis. Cx trafficking and endocytosis need interactions with a large number of signaling and scaffolding proteins. We demonstrate herein that Cx43-GFP gap junction plaque endocytosis was blocked in cells transfected by the dominant-negative form of dynamin2 (Dyn2K44A) and by dynasore, an inhibitor of dynamin GTPase activity, which reduced the association between dynamin2 and Cx43. Our data also reveal that recruitment of the GTPase at the plasma membrane and its activation by c-Src are key events for Cx43 internalization. In addition they show that dynamin2 participated in internalization and degradation of the gap junction plaque but also in recycling of Cx43 to the plasma membrane through respectively Rab5/Rab7 and Rab11 pathways. These results demonstrate for the first time that dynamin2 is a new Cx partner and report an innovating mechanistic model by which dynamin2 may control Cx43 gap junction plaque invagination, endocytosis, recycling and degradation. These processes are magnified in response to carcinogen exposure underlining their potential importance during carcinogenesis.
Databáze: OpenAIRE
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