Cerebrospinal Fluid Aβ42/Aβ40 as a Means to Limiting Tube- and Storage-Dependent Pre-Analytical Variability in Clinical Setting

Autor: Brigitte Dousset, Eliane Albuisson, Gasshan Watfa, Catherine Malaplate-Armand, Thérèse Rivasseau Jonveaux, Allan Kolodziej, Christelle Gervaise-Henry, Jean-Luc Olivier
Přispěvatelé: Biochimie – Biologie moléculaire et Nutrition [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Service de Gériatrie [CHRU Nancy], INSERM, l'Institut national de la santé et de la recherche médicale (INSERM), Ministère de la Santé et de la Recherche, Institut Élie Cartan de Lorraine (IECL), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), ESPRI-Biobase [CHRU Nancy] (Unité fonctionnelle de la plateforme d’aide à la recherche clinique), Composés Alimentaires : Biofonctionnalités et risques Neurotoxiques (CALBINOTOX), Université de Lorraine (UL), Olivier, Jean Luc
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
[SDV]Life Sciences [q-bio]
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Routine practice
pre-analytical standardization
freeze-thaw cycles
0302 clinical medicine
Cerebrospinal fluid
Freezing
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Aged
80 and over
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
[SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
Pre analytical
General Neuroscience
[SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology
General Medicine
Limiting
Middle Aged
Alzheimer's disease
[SDV] Life Sciences [q-bio]
Psychiatry and Mental health
Clinical Psychology
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
medicine.medical_specialty
tube
Urology
tau Proteins
Aβ42/Aβ40 ratio
Polypropylenes
cerebrospinal fluid
Specimen Handling
03 medical and health sciences
medicine
Humans
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Aged
Amyloid beta-Peptides
business.industry
Aβ40 peptide
Aβ42 peptide
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Peptide Fragments
Surgery
030104 developmental biology
Geriatrics and Gerontology
business
030217 neurology & neurosurgery
Biomarkers
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Journal of Alzheimer's Disease
Journal of Alzheimer's Disease, IOS Press, 2017, 57 (2), pp.437-445. ⟨10.3233/JAD-160865⟩
Journal of Alzheimer's Disease, 2017, 57 (2), pp.437-445. ⟨10.3233/JAD-160865⟩
ISSN: 1387-2877
Popis: Background Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers have recently been included in the criteria for AD diagnosis. Unfortunately, their wider use in routine and interpretation require more standardization, particularly for the pre-analytical steps. In particular, amyloid-β (Aβ)42 peptide measurement is strongly influenced by the type of collection tube and by repeated freeze/thaw cycles. Objective The objectives of this study were to compare, in clinical setting, the impact of collection tubes and the repetition of freeze/thaw cycles on Aβ42 and Aβ40 concentrations and consequently determine if the Aβ42/Aβ40 ratio could resolve the diagnosis difficulties related to these pre-analytical parameters. Methods CSF from 35 patients was collected in different polypropylene (PP) and stored at - 80°C after sampling. For CSF collected in the reference tube, three successive freeze-thaw cycles were done. Aβ42 and Aβ40 concentrations were determined in each condition in order to calculate the Aβ42/Aβ40 ratio. Results Our results showed that CSF Aβ42 and Aβ40 values were significantly different according to the type of collection tube and the number of freeze/thaw cycles. Although the calculation of the Aβ42/Aβ40 ratio eliminated the effect of PP tube-dependent variation, this was not the case for freeze-thaw cycle-associated variation. Conclusion The use of Aβ42/Aβ40 ratio rather than Aβ42 alone could contribute toward pre-analytical standardization, thus allowing the general use of CSF AD biomarkers in routine practice.
Databáze: OpenAIRE
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