Wnt/β-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells

Autor: Bisei Ohkawara, Denitsa Docheva, Kinji Ohno, Naoki Ishiguro, Tadahiro Sakai, Yasuzumi Kishimoto, Mikako Ito, Akio Masuda, Chisa Shukunami
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Cell signaling
Gene Expression
Smad Proteins
Signal transduction
Biochemistry
Tendons
Rats
Sprague-Dawley
Animal Cells
Transforming Growth Factor beta
Medicine and Health Sciences
Basic Helix-Loop-Helix Transcription Factors
Post-Translational Modification
Phosphorylation
Wnt Signaling Pathway
beta Catenin
Regulation of gene expression
Multidisciplinary
Stem Cells
Wnt signaling pathway
Signaling cascades
musculoskeletal system
Tendon
Cell biology
medicine.anatomical_structure
Connective Tissue
Medicine
Anatomy
Cellular Types
Research Article
musculoskeletal diseases
medicine.medical_specialty
Signal Inhibition
Science
SMAD signaling
Biology
03 medical and health sciences
Downregulation and upregulation
Internal medicine
medicine
Genetics
Animals
Humans
RNA
Messenger
Homeodomain Proteins
Staining and Labeling
Regeneration (biology)
Mesenchymal stem cell
Biology and Life Sciences
Proteins
Membrane Proteins
Mesenchymal Stem Cells
030104 developmental biology
Endocrinology
Biological Tissue
TGF-beta signaling cascade
Gene Expression Regulation
Collagens
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 7, p e0182051 (2017)
ISSN: 1932-6203
Popis: After tendon injuries, biomechanical properties of the injured tendon are not fully recovered in most cases. Modulation of signaling pathways, which are involved in tendon development and tendon repair, is one of attractive modalities to facilitate proper regeneration of the injured tendon. The roles of TGF-β signaling in tendon homeostasis and tendon development have been elucidated. In contrast, the roles of Wnt/β-catenin signaling in tendon remain mostly elusive. We found that the number of β-catenin-positive cells was increased at the injured site, suggesting involvement of Wnt/β-catenin signaling in tendon healing. Activation of Wnt/β-catenin signaling suppressed expressions of tenogenic genes of Scx, Mkx, and Tnmd in rat tendon-derived cells (TDCs) isolated from the Achilles tendons of 6-week old rats. Additionally, activation of Wnt/β-catenin reduced the amounts of Smad2 and Smad3, which are intracellular mediators for TGF-β signaling, and antagonized upregulation of Scx induced by TGF-β signaling in TDCs. We found that Wnt/β-catenin decreased Mkx and Tnmd expressions without suppressing Scx expression in Scx-programmed tendon progenitors. Our studies suggest that Wnt/β-catenin signaling is a repressor for tenogenic gene expressions.
Databáze: OpenAIRE
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