Evaluation of biological activities of a groundnut (Apios americana Medik) extract containing a novel isoflavone
| Autor: | Yoshihisa Hagihara, Mayumi Stowe, Etsuo Niki, Kazuhiro Nara, Mizuki Takashima, Yasukazu Yoshida, Masanori Horie |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
Antioxidants Analytical Chemistry chemistry.chemical_compound Cell Line Tumor Gene expression Extracellular Humans Heme chemistry.chemical_classification biology Plant Extracts Chemistry food and beverages Fabaceae General Medicine Glutathione Isoflavones Apios biology.organism_classification Enzyme Biochemistry Radical initiator Female Food Science |
| Zdroj: | Food Chemistry. 138:298-305 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2012.10.100 |
| Popis: | Groundnut (Apios americana Medik) contains a novel isoflavone, genistein-7-O-gentiobioside. In the present study, we examined the biological activities of an alcohol extract of groundnut containing genistein-7-O-gentiobioside as the main component. Although the groundnut extract by itself did not show antioxidative activity, it drove the antioxidative system in cells. Pretreatment of human breast carcinoma MCF-7 cells for 24 h with the groundnut extract and soybean isoflavone increased gene expression of heme oxygenase-1 (HO-1), a major antioxidative stress enzyme. These groundnut extract-treated cells showed antioxidative activity against free radicals derived from a radical initiator. Pretreatment of cells with 100 μg/mL groundnut extract prevented the depletion of glutathione by the radical initiator; however, treatment with 100 μg/mL of soybean isoflavone injured the cell membrane, indicating that glutathione might be released to the extracellular environment. These results suggest that the groundnut extract had isoflavone-like activity. Like soybean, groundnuts are a good source of isoflavones. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect