Spectrum of metabolic-based resistance to DDT and pyrethroids in Anopheles gambiae s.l. populations from Cameroon
| Autor: | L. Manga, Etienne Fondjo, Fabrice Chandre, Pierre Guillet, J.C. Toto, Josiane Etang |
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| Rok vydání: | 2007 |
| Předmět: |
Insecticides
Anopheles gambiae DDT Mixed Function Oxygenases Microbiology Insecticide Resistance Toxicology chemistry.chemical_compound Anopheles gambiae S Anopheles Pyrethrins parasitic diseases Animals Cameroon Ecology Evolution Behavior and Systematics Glutathione Transferase chemistry.chemical_classification Pyrethroid Geography Ecology biology Strain (chemistry) Esterases Glutathione biology.organism_classification Metabolic detoxification Enzyme assay Enzyme chemistry biology.protein |
| Zdroj: | Journal of Vector Ecology. 32:123-133 |
| ISSN: | 1081-1710 |
| DOI: | 10.3376/1081-1710(2007)32[123:somrtd]2.0.co;2 |
| Popis: | Some populations of Anopheles gambiae s.l. from Cameroon were reported to develop resistance to DDT or pyrethroids but were free of the kdr mutation "Leucine-Phenylalanine" (Leu-Phe). This study reports on the metabolic activity of non-specific esterases (NSEs), mixed function oxidases (MFOs), and glutathione S-transferases (GSTs), three enzyme systems commonly involved in insecticide resistance. Biochemical assays were performed in DDT or pyrethroid-resistant populations of An. gambiae s.l. from Douala, Mbalmayo, Pitoa, and Simatou neighborhoods. Enzyme activity was compared to the Kisumu-susceptible reference strain using the Mann-Whitney test. Most of the tested samples had elevated NSE activity (P0.02). The Douala sample evenly displayed elevated GST activity (P0.001), while high MFO level was recorded in the Pitoa sample (P0.001). MFO or GST levels were sometimes lower or similar to that of the Kisumu strain. These results suggest metabolic detoxification is a major DDT or pyrethroid resistance mechanism and emphasize the need for further investigations on An. gambiae s.l. resistance mechanisms in Cameroon. |
| Databáze: | OpenAIRE |
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