Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes
Autor: | Hyuncheol Oh, Joung Han Yim, Jae Hak Sohn, Dong-Sung Lee, Linsha Dong, Thao Quyen Cao, Tai Kyoung Kim, Wonmin Ko, Hye Jin Kim, Hwan Lee, Zhiming Liu, Youn-Chul Kim |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
medicine.drug_class
ICAM-1 QH301-705.5 Alternariol HO-1 Catalysis Anti-inflammatory CCL5 NF-κB Inorganic Chemistry chemistry.chemical_compound medicine Secretion Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Chemistry HaCaT Organic Chemistry General Medicine Molecular biology Antarctic fungi Computer Science Applications Heme oxygenase inflammation Tumor necrosis factor alpha |
Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 9674, p 9674 (2021) International Journal of Molecular Sciences; Volume 22; Issue 18; Pages: 9674 |
ISSN: | 1661-6596 1422-0067 |
Popis: | Chemical investigation of the Antarctic fungi Pleosporales sp. SF-7343 revealed four known secondary fungal metabolites: alternate C (1), altenusin (2), alternariol (3), and altenuene (4). The compound structures were identified primarily by NMR and MS analyses. Atopic dermatitis, an inflammatory disease, is driven by the abnormal activation of T helper (Th) 2 cells and barrier dysfunction. We attempted to identify the anti-inflammatory components of SF-7343. Initial screening showed that compounds 1 and 3 inhibited the secretion of interleukin-8 and -6 in tumor necrosis factor-α/interferon-γ-treated HaCaT cells, and these compounds also showed inhibitory effects on CCL5 and CCL22. Compounds 1 and 3 also downregulated the protein expression levels of intercellular adhesion molecule-1 and upregulated the expression of filaggrin and involcurin. The mechanism study results showed that compounds 1 and 3 inhibited nuclear translocation of nuclear factor-kappa B p65 and the phosphorylation of STAT1 and STAT3. Compound 1, but not compound 3, significantly promoted the expression of heme oxygenase (HO)-1. The effects of compound 1 were partly reversed by co-treatment with a HO-1 inhibitor, tin protoporphyrin IX. Taken together, this study demonstrates the potential value of Antarctic fungal strain SF-7343 isolates as a bioresource for bioactive compounds to prevent skin inflammation. |
Databáze: | OpenAIRE |
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