A Simple Method to Obtain Low Density Marrow Cells for Human Marrow Transplantation
| Autor: | E. Koekman, J Vet, T. de Witte, F. Preyers, A. Plas, J. Wessels |
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| Rok vydání: | 1987 |
| Předmět: |
Pathology
medicine.medical_specialty Myeloid Density gradient business.industry Povidone Hematology General Medicine T lymphocyte In vitro Leukemia Lymphoid Specimen Handling medicine.anatomical_structure Nucleated cell Centrifugation Density Gradient medicine Humans Centrifugation Bone marrow business Percoll Bone Marrow Transplantation |
| Zdroj: | Vox Sanguinis. 53:221-227 |
| ISSN: | 1423-0410 0042-9007 |
| DOI: | 10.1111/j.1423-0410.1987.tb05070.x |
| Popis: | Removal of more than 99% of the erythrocytes and 74% of the nucleated cells from marrow grafts was achieved by density floatation separation in Percoll gradients with a density of 1.070 g/ml in eight 250-ml tubes, containing up to 3 X 10(9) nucleated cells per gradient. More than 90% of the myeloid and erythroid progenitor cells were recovered in the low density fraction. It appeared mandatory to use a centrifuge with the possibility of a gradual acceleration and deceleration. Twenty-five patients received a marrow graft from a histocompatible sibling after additional lymphocyte depletion by counterflow centrifugation, and 5 patients with T lymphoblastic malignancies received an autograft after in vitro purging with immunotoxins. All evaluable patients engrafted within normal limits, except 1 patient with an autoimmune pancytopenia who responded to steroids and 1 patient with a CMV infection. Four patients died too early for complete evaluation. The described separation method is easy, cheap and requires only 2 h for the complete processing of a marrow graft. |
| Databáze: | OpenAIRE |
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