Efficacy of Syringomycin E in a Murine Model of Vaginal Candidiasis
| Autor: | April A. Wanstrom, Jon Y. Takemoto, Kevin N. Sorensen, S. D. Allen |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Antifungal Agents
Ratón Biology Pharmacology Peptides Cyclic Dosage form Microbiology Mice Vaginal disease Drug Discovery PEG ratio medicine Animals Clotrimazole Candidiasis Vulvovaginal Chromatography High Pressure Liquid Drug Carriers Mice Inbred ICR Dose-Response Relationship Drug Disease Models Animal medicine.anatomical_structure Liver Vagina Female Intravaginal administration Drug carrier medicine.drug |
| Zdroj: | The Journal of Antibiotics. 51:743-749 |
| ISSN: | 1881-1469 0021-8820 |
| DOI: | 10.7164/antibiotics.51.743 |
| Popis: | Syringomycin E (SR-E), a new antifungal produced by the bacterium Pseudomonas syringae pv. syringae, was evaluated in a murine vaginal candidiasis model. In one study, mice were treated intravaginally b.i.d. for 4 days with drug carrier, SR-E 2% in either PEG-400 or PEG-ointment, or 1% clotrimazole as a positive control. Quantitative vaginal cultures were taken prior to treatment on day 1 and on days 5, 6, and 7. Both formulations showed a reduction of yeast colonization in the vaginas on day 5 (Por =0.06 and Por =0.03 for SR-E/PEG-400 and SR-E/PEG ointment, respectively) and SR-E/PEG ointment reduced the colonization on day 7 (Por =0.06) when compared to carrier treated controls. In a second study, SR-E was formulated in Aquaphor at three higher concentrations of SR-E [3%, 6%, or 12% (w/v)]. SR-E showed dose-dependent efficacy. The 3% dose showed no effect while the 6% and 12% doses reduced the number of yeasts. The 12% dose showed a significant reduction on days 5 (Por =0.01), 6 (Por =0.06), and 7 (Por =0.03) when compared with the drug carrier controls and on day 5 was more effective than clotrimazole (Por =0.03). Clotrimazole did not significantly reduce the yeasts in the vagina until days 6 (Por =0.01) and 7 (Por =0.01) when compared to the drug carrier controls. No vaginal inflammatory response was evident by histological examination in uninfected animals treated with SR-E. No SR-E could be detected in plasma, kidney, or liver. SR-E (12%) was an effective treatment when compared to 1% clotrimazole. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|