Influence of incubation time and action of fluid flow on SPEV cell adhesion strength to a glass substrate
| Autor: | O. V. Timofeyeva, A. F. Todrin, T. P. Petrenko |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Substrate (chemistry) 02 engineering and technology Surfaces and Interfaces General Chemistry 021001 nanoscience & nanotechnology Surfaces Coatings and Films Incubation period Adhesion strength 03 medical and health sciences 030104 developmental biology Mechanics of Materials Materials Chemistry Fluid dynamics Biophysics 0210 nano-technology Cell adhesion strength incubation time flow action time initial shear stress critical radius critical shear stress cell projection area |
| Zdroj: | Journal of Adhesion Science and Technology. 32:1838-1848 |
| ISSN: | 1568-5616 0169-4243 |
| DOI: | 10.1080/01694243.2018.1448500 |
| Popis: | The results of study of SPEV cell adhesion to the glass substrate that were obtained with a radial-flow chamber shown that in some cases the cells which were incubated on the substrate for a longer time were more strongly attached to it, and under certain conditions such cells detached easier than cells with a shorter incubation time. For example, when the channel height was decreased more of cells were detached from substrate with increasing incubation time. Besides an increase of flow action time on the cells increased an amount of detached cells, and an increase of initial shear stress resulted in decrease an amount of cells which detached from the surface regardless of incubation time. During each experiment more spread cells detached from the substrate earlier than the more spherical one. When both the flow rate and channel height were reduced in the radial-flow chamber, but the initial shear stress was the same as before the changes of these parameters, then the critical radius, the critical shear stress and the time of reach these critical values sharply decreased. In addition, in this case, the area of the projection of cells remaining on the substrate also decreased. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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