Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis
Autor: | Jennifer Klemm, Martina Lachnit, Nils Kröger, Matthias T. Buhmann, Nicole Poulsen |
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Rok vydání: | 2019 |
Předmět: |
Diatoms
0303 health sciences biology Proteome 030306 microbiology Biofouling Surface Properties fungi Algal Proteins Biofilm Marine invertebrates Articles biology.organism_classification Proteomics General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Diatom Botany Cell Adhesion Identification (biology) Adhesive Amphora coffeaeformis General Agricultural and Biological Sciences Bacteria 030304 developmental biology |
Zdroj: | Philos Trans R Soc Lond B Biol Sci |
ISSN: | 1471-2970 |
Popis: | Throughout all kingdoms of life, a large number of adhesive biomolecules have evolved to allow organisms to adhere to surfaces underwater. Proteins play an important role in the adhesion of numerous marine invertebrates (e.g. mussels, sea stars, sea urchins) whereas much less is known about the biological adhesives from marine plants, including the diatoms. Diatoms are unicellular microalgae that together with bacteria dominate marine biofilms in sunlit habitats. In this study we present the first proteomics analyses of the diatom adhesive material isolated from the tenacious fouling species Amphora coffeaeformis . We identified 21 proteins, of which 13 are diatom-specific. Ten of these proteins share a conserved C-terminal domain, termed GDPH domain, which is widespread yet not ubiquitously present in all diatom classes. Immunofluorescence localization of a GDPH domain bearing protein (Ac629) as well as two other proteins identified in this study (Ac1442, Ac9617) demonstrated that these are components of the adhesive trails that are secreted by cells that glide on surfaces. This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’. |
Databáze: | OpenAIRE |
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