Method comparison for analyzing wound healing rates
| Autor: | Bingyun Sun, Oluwafemi S. Akintola, Prabhpreet K. Dhillon, Xinyin Li, Jurgen T. Sanes |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Wound Healing Chinese hamster ovary cell Cell migration Regression rate CHO Cells Cell Biology Anatomy Biology Tissue repair Biochemistry 03 medical and health sciences Cricetulus 030104 developmental biology Method comparison Cell Movement Cricetinae Animals Biological Assay Wound healing Molecular Biology Cell Proliferation Biomedical engineering |
| Zdroj: | Biochemistry and Cell Biology. 95:450-454 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/bcb-2016-0163 |
| Popis: | Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: (I) direct rate average; (II) regression rate average; and (III) average distance regression rate. Using Chinese hamster ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers, and type II analysis is more sensitive to outliers. We hope this study can help researchers to better use this simple yet effective assay. |
| Databáze: | OpenAIRE |
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