Mechanical stimulation of bone cells using fluid flow

Autor:	Huesa, C., Bakker, A.D., Helfrich, M.H., Ralston, S.H.
Přispěvatelé:	Orale Celbiologie (ORM, ACTA)
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	chemistry.chemical_compound Materials science chemistry Cell culture Monolayer Bone cell Fluid dynamics Pulsatile flow Stimulation Anatomy In vitro Nitric oxide Biomedical engineering
Zdroj:	Bone Research Protocols, 573-592 ISSUE=2nd;STARTPAGE=573;ENDPAGE=592;TITLE=Bone Research Protocols Methods in Molecular Biology ISBN: 9781617794148
ISSN:	1064-3745
DOI:	10.1007/978-1-61779-415-5_36
Popis:	This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffb9251c6493a69a363513cb33bad3f1 https://doi.org/10.1007/978-1-61779-415-5_36 Zobrazit plný text záznamu