CDR3 clonotype and amino acid motif diversity of BV19 expressing circulating human CD8 T cells
| Autor: | Wendy Demos, Jack Gorski, Teresa Janczak, Maryam Yassai, Elena N. Naumova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Receptors Antigen T-Cell alpha-beta Amino Acid Motifs Immunology T-Cell Antigen Receptor Specificity CD8-Positive T-Lymphocytes Biology Peripheral blood mononuclear cell Article Epitope DNA sequencing Viral Matrix Proteins 03 medical and health sciences 0302 clinical medicine HLA-A2 Antigen Influenza Human Humans Immunology and Allergy Cytotoxic T cell Nucleotide Genetics chemistry.chemical_classification Repertoire T-cell receptor General Medicine Complementarity Determining Regions Peptide Fragments Clone Cells Amino acid 030104 developmental biology chemistry Influenza A virus Blood Circulation Immunologic Memory 030215 immunology |
| Zdroj: | Human Immunology. 77:137-145 |
| ISSN: | 0198-8859 |
| Popis: | Generating a detailed description of human T cell repertoire diversity is an important goal in the study of human immunology. The circulation is the source of most T cells used for studies in humans. Here we use high throughput sequencing of TCR BV19 transcripts from CD8 T cells derived from unmanipulated PBMC from an older HLA-A2 individual to provide a quantitative and qualitative description of the clonotypic CDR3 nucleotide and amino acid composition of the TCR β-chain from this subset of circulating CD8 T cells. Aggregated samples from six time points spanning ∼1.5 years were analyzed to smooth possible temporal fluctuation. BV19 encompasses the well studied RS-encoding clonotypes involved in recognition of the M1(58-66) epitope from influenza A in HLA-A2 individuals. The clonotype distribution was diverse, complex and self-similar. The amino acid composition was generally skewed in favor of glycines and there were specific amino acids observed at higher frequency at the NDN start position. The motif repertoire distribution was also diverse, complex and self-similar with respect to CDR3 length, NDN start and length. |
| Databáze: | OpenAIRE |
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