In vivo exposure to bicarbonate/lactate- and bicarbonate-buffered peritoneal dialysis fluids improves ex vivo peritoneal macrophage function
| Autor: | Nicholas Topley, Ruth K. Mackenzie, Kewei Pu, Clifford J. Holmes, John D. Williams, Gerald A. Coles, Suzanne Jones, Andrew Moseley, Ruth Argyle, Dirk Faict |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment Microgram Bicarbonate Peritonitis Peritoneal dialysis chemistry.chemical_compound Peritoneal Dialysis Continuous Ambulatory Peritoneum In vivo Dialysis Solutions Internal medicine medicine Humans Lactic Acid Dialysis Aged Tumor Necrosis Factor-alpha business.industry Zymosan Hydrogen-Ion Concentration Macrophage Activation Middle Aged Bicarbonates medicine.anatomical_structure Endocrinology chemistry Nephrology Macrophages Peritoneal Female business Ex vivo |
| Zdroj: | American Journal of Kidney Diseases. 35:112-121 |
| ISSN: | 0272-6386 |
| DOI: | 10.1016/s0272-6386(00)70309-x |
| Popis: | The impact on peritoneal macrophage (PMO) function of acidic lactate-buffered (Lac-PDF [PD4]; 40 mmol/L of lactate; pH 5.2) and neutral-pH, bicarbonate-buffered (TB; 38 mmol/L of bicarbonate; pH 7. 3) and bicarbonate/lactate-buffered (TBL; 25 mmol/L of bicarbonate/15 mmol/L of lactate; pH 7.3) peritoneal dialysis fluids (PDFs) was compared during a study of continuous therapy with PD4, TB, or TBL. During a run-in phase of 6 weeks when all patients (n = 15) were treated with their regular dialysis regimen with Lac-PDF, median PMO tumor necrosis factor alpha (TNFalpha) release values were 203.6, 89.9, and 115.5 pg TNFalpha/10(6) PMO in the patients subsequently randomized to the PD4, TB, and TBL treatment groups, respectively. Median stimulated TNFalpha values (serum-treated zymosan [STZ], 10 microgram/mL) were 1,894.6, 567.3, and 554.5 pg TNFalpha/10(6) PMO in the same groups, respectively. During the trial phase of 12 weeks, when the three groups of patients (n = 5 per group) were randomized to continuous treatment with PD4, TB, or TBL, median constitutive TNFalpha release values were 204.7, 131.4, and 155.4 pg TNFalpha/10(6) PMO, respectively. Stimulated TNFalpha values (STZ, 10 microgram/mL) were 1,911, 1,832, and 1,378 pg TNFalpha/10(6) PMO in the same groups, respectively. Repeated-measures analysis of variance comparing the run-in phase with the trial phase showed that PMO TNFalpha release was significantly elevated in patients treated with both TB (P = 0.040) and TBL (P = 0.014) but not in patients treated with Lac-PDF (P = 0. 795). These data suggest that patients continuously exposed to bicarbonate- and bicarbonate/lactate-buffered PDFs might have better preserved PMO function and thus improved host defense status. |
| Databáze: | OpenAIRE |
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