Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Autor: Jing Li, Jan Haug Anonsen, Eeva-Liisa Eskelinen, Sigurdur Gudmundsson, Suresh Kumar, Vojo Deretic, Chunyan Ye, Brett S. Phinney, Sandeep Pallikkuth, Keith A. Lidke, Alf Håkon Lystad, Graham S. Timmins, Tor Erik Rusten, Anne Simonsen, Steven B. Bradfute, Michelle Salemi, Ashish Jain, Michal H. Mudd, Aurore Claude-Taupin, Karthikeyan Tangavelou, Ruheena Javed, Lian-Wang Guo
Jazyk: angličtina
Rok vydání: 2021
Předmět:
coronavirus
sigma
ATG16L1
Golgi Apparatus
Endoplasmic Reticulum
Medical and Health Sciences
Membrane Fusion
Synaptotagmins
Phagosomes
Receptors
Golgi
Lung
Microscopy
Tumor
Microscopy
Confocal
Qa-SNARE Proteins
Vesicle
Biological Sciences
Cell biology
Infectious Diseases
Membrane
Confocal
symbols
autophagy
Endosome
Endosomes
Syntaxin 17
Biology
General Biochemistry
Genetics and Molecular Biology
Article
Cell Line
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Vaccine Related
Atg8ylation
symbols.namesake
Biodefense
Cell Line
Tumor
Autophagy
Humans
Receptors
sigma
FIP200
endosome
Pre-autophagosomal structure
SARS-CoV-2
Prevention
Autophagosomes
Lipid bilayer fusion
COVID-19
Pneumonia
Golgi apparatus
Emerging Infectious Diseases
HEK293 Cells
Hela Cells
CRISPR-Cas Systems
Biogenesis
Developmental Biology
HeLa Cells
Zdroj: Cell
Cell, vol 184, iss 24
Popis: The biogenesis of mammalian autophagosomes remains to be fully defined. Here, we used cellular and invitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment. The autophagic precursors emerge through fusion of FIP200 vesicles, derived from the cis-Golgi, with endosomally derived ATG16L1 membranes to generate a hybrid pre-autophagosomal structure, HyPAS. A previously unrecognized apparatus defined here controls HyPAS biogenesis and mammalian autophagosomal precursor membranes. HyPAS can be modulated by pharmacological agents whereas its formation is inhibited upon severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or by expression of SARS-CoV-2nsp6. These findings reveal the origin of mammalian autophagosomal membranes, which emerge via convergence of secretory and endosomal pathways, and show that this process is targeted by microbial factors such as coronaviral membrane-modulating proteins.
Databáze: OpenAIRE
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