Application of PLGA/FGF-2 coaxial microfibers in spinal cord tissue engineering: an in vitro and in vivo investigation
| Autor: | Cristian Euzebio Teixeira, Ágata Paim, Roland A. Fleck, Laura E. Sperling, Bruno Alcântara, Gema Vizcay-Barrena, Patricia Pranke, Karina Pires Reis |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryology Biomedical Engineering 02 engineering and technology PC12 Cells 03 medical and health sciences chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Tissue engineering In vivo Materials Testing medicine Animals Spinal cord injury Spinal Cord Injuries Tissue Engineering Tissue Scaffolds Nestin 021001 nanoscience & nanotechnology medicine.disease Spinal cord Rats PLGA 030104 developmental biology medicine.anatomical_structure Spinal Cord chemistry Systemic administration Liberation 0210 nano-technology Biomedical engineering |
| Zdroj: | Regenerative Medicine. 13:785-801 |
| ISSN: | 1746-076X 1746-0751 |
| DOI: | 10.2217/rme-2018-0060 |
| Popis: | Aim: Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but is easily degradable and presents collateral effects in systemic administration. In order to address the stability issue and avoid the systemic effects, FGF-2 was encapsulated into core–shell microfibers by coaxial electrospinning and its in vitro and in vivo potential were studied. Materials & methods: The fibers were characterized by physicochemical and biological parameters. The scaffolds were implanted in a hemisection SCI rat model. Locomotor test was performed weekly for 6 weeks. After this time, histological analyses were performed and expression of nestin and GFAP was quantified by flow cytometry. Results: Electrospinning resulted in uniform microfibers with a core–shell structure, with a sustained liberation of FGF-2 from the fibers. The fibers supported PC12 cells adhesion and proliferation. Implanted scaffolds into SCI promoted locomotor recovery at 28 days after injury and reduced GFAP expression. Conclusion: These results indicate the potential of these microfibers in SCI tissue engineering. [Formula: see text] |
| Databáze: | OpenAIRE |
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