Experimental Visceral Leishmaniasis in the Opossum
| Autor: | C. E. Greene, Kenneth S. Latimer, M. R. White, Willie L. Chapman, Hanson Wl |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
040301 veterinary sciences 030231 tropical medicine Population Leishmania donovani Antibodies Protozoan Spleen Neutropenia Lymphocyte Activation 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Opossum medicine Animals education Blood Coagulation education.field_of_study General Veterinary biology Macrophages Opossums 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Blood Cell Count Disease Models Animal Visceral leishmaniasis medicine.anatomical_structure Immunology biology.protein Leishmaniasis Visceral Female Lymph Antibody |
| Zdroj: | Veterinary Pathology. 26:314-321 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098588902600405 |
| Popis: | Adult wild-trapped opossums were infected with Leishmania donovani (Khartoum strain, WR 378) and evaluated as an animal model of visceral leishmaniasis. All infected opossums died within 32 days. Loss of body fat, hepatomegaly, and petechiae of skin and abdominal musculature were seen at necropsy. Microscopically, numerous amastigote-laden macrophages were seen in histologic sections of liver, spleen, and lymph nodes; fewer parasite-laden macrophages were in the bronchial-associated lymphoid tissues and renal glomeruli. Hematological findings included thrombocytopenia (terminal), neutropenia, and lymphopenia. Blood lymphocyte blastogenesis in response to concanavalin A and phytohemagglutinin was decreased markedly at day 24 post-infection (PI). Serum antibodies (1 : 40 dilution) to promastigotes of L. donovani were detected in five of eight infected opossums tested on days 10 and 24 PI. Total bilirubin concentrations and alanine aminotrans- ferase and aspartate aminotransferase activities were increased after day 25 PI. Activated partial thromboplastin times and one-stage prothrombin times were prolonged before death. Concurrently, factors V, VIII, and XI1 activities were decreased. Visceral leishmaniasis, caused by several subspecies of a protozoan Leishmania donovani,22 is a severe, chronic, debilitating disease which affects people and animals worldwide. Several different animal species including rodents,I6 nonhuman primate^,^,^^,^^ and dogs2* have been used to study visceral leishmaniasis e~perimentally.~ The oppossum is susceptible to vis- ceral lei~hmaniasis'~ and rapidly develops fatal system- ic disease. Advantages of using the opossum as an animal mod- el for visceral leishmaniasis include its availability in the wildlife population and an adequate body size for repeated hematologic sampling. Disadvantages in- clude the necessity for physical or chemical restraint and the lack of commercially available laboratory- reared animals. The purposes of this study were to examine the mor- phologic, clinicopathologic, and immunologic findings in opossums infected with L. donovani and to deter- mine if the opossum was a suitable animal model to study visceral leishmaniasis. |
| Databáze: | OpenAIRE |
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