Antisera Against the Indolealkylamines: Tryptophan, 5-Hydroxytryptophan, 5-Hydroxytryptamine, 5-Methoxytryptophan, and 5-Methoxytryptamine Tested by an Enzyme-Linked Immunosorbent Assay Method
| Autor: | Michel Geffard, Josette Dulluc, Anne-Marie Rock |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Tryptamine
Serotonin Enzyme-Linked Immunosorbent Assay Biochemistry 5-Hydroxytryptophan 5-Methoxytryptamine Cellular and Molecular Neuroscience chemistry.chemical_compound Antibody Specificity medicine Bovine serum albumin Serum Albumin Antiserum chemistry.chemical_classification biology Immune Sera Tryptophan Serum Albumin Bovine Human serum albumin Molecular biology Tryptamines Enzyme chemistry Glutaral Spectrophotometry biology.protein Antibody Haptens medicine.drug Conjugate |
| Zdroj: | Journal of Neurochemistry. 44:1221-1228 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1985.tb08747.x |
| Popis: | Antisera were raised against tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-methoxytryptophan, and 5-methoxytryptamine, by conjugating each molecule to bovine serum albumin and to human serum albumin via glutaraldehyde, in such a way as to preserve the original part. Antibody specificity was tested with the enzyme-linked immunosorbent assay method. The specificity of each anti-indolealkylamine-glutaraldehyde antibody was established with competition experiments by using an adsorbed immunogenic conjugate and indolealkylamines either free or conjugated with poly-L-lysine. The nonconjugated compounds were poorly recognized. In the same way, the nonreduced conjugates always appeared less immunoreactive than the reduced ones. Calculated from the specificity study of each antiserum, the cross-reactivity ratios were found to be smallest for the most immunoreactive conjugates. Thus, a specific immune response was defined for each compound belonging to the same metabolic pathway. |
| Databáze: | OpenAIRE |
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