A rapid solid-phase rosette-inhibition assay for the detection of Fc receptor (FcRIII)-blocking alloantibodies
| Autor: | Katherine A. Leader, Peter T. Klouda, Godfrey J. Laundy, Benjamin A. Bradley, Andrew G. Hadley, Adrian Smith |
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| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Rosette Formation Immunology Fc receptor Rosette inhibition test Receptors Fc Immunoglobulin E Binding Competitive Cell Line HLA Antigens Isoantibodies HLA-A2 Antigen Blocking antibody Humans Immunology and Allergy Cell Line Transformed B-Lymphocytes biology Rosette (schizont appearance) Virology Molecular biology Rosette formation Immunoglobulin G biology.protein Antibody Fc fragment |
| Zdroj: | Immunology Letters. 27:215-220 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/0165-2478(91)90154-3 |
| Popis: | A procedure for the detection of FcR-blocking alloantibodies is described which uses human B lymphocytes immobilised on plastic by poly-L-lysine. Antibodies which inhibited rosette formation between B lymphocytes or Daudi cells and ox erythrocytes coated with rabbit antibodies (EA) were detected in 10 out of 10 sera containing anti-HLA A2 antibodies and 3 out of 3 sera containing anti-HLA class II antibodies. Inhibition of rosette formation (EAI activity) was mediated by protein G-separated IgG. Analysis of rosette formation using these 13 sera and lymphocytes from 39 donors revealed that the degree of inhibition was bimodal; most sera were either clearly inhibitory or non-inhibitory in the assay. However, there was no correlation between inhibition of rosette formation (EAI activity) and lymphocytotoxicity. Four pairs of sera showed similar patterns of reactivity (r greater than 0.6, p less than 0.01; 2 x 2 chi-square test), and cells from 2 donors showed antithetical reactions with 12 of 13 sera (r = -0.86, p less than 0.001). These data suggest that the solid-phase rosette inhibition assay is a rapid and reproducible means of detecting antibodies reactive with non-HLA class I or class II antigens on human B cells. |
| Databáze: | OpenAIRE |
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