An optimal LC-MS/MS method for determination of azithromycin in white blood cells: application to pediatric samples
| Autor: | Evelyne Jacqz-Aigrain, Tiphaine Legrand, Sailesh Kotecha, Christophe Junot, Valery Elie, Alain Pruvost |
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| Rok vydání: | 2014 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Adolescent Clinical Biochemistry Context (language use) Cell Count Azithromycin Analytical Chemistry Limit of Detection Tandem Mass Spectrometry Lc ms ms medicine Leukocytes Humans General Pharmacology Toxicology and Pharmaceutics Child Whole blood Chromatography Chemistry Selected reaction monitoring Infant Newborn Analytic Sample Preparation Methods Infant Reproducibility of Results General Medicine Medical Laboratory Technology Child Preschool medicine.drug Chromatography Liquid |
| Zdroj: | Bioanalysis. 6(17) |
| ISSN: | 1757-6199 |
| Popis: | Background: Studies suggest that particular antimicrobial and anti-inflammatory properties of azithromycin (AZM) can be related to its extensive accumulation in white blood cells (WBCs). However, available methods for determination of AZM in WBCs require large blood volumes unsuited to a pediatric context. Therefore, an LC-MS/MS method was developed for determination of AZM in WBCs. Results: WBCs were isolated from 500 µl of whole blood by lysing red blood cells. Then, lysis of WBCs was performed with methanol/buffer containing AZM-d3-13C as internal standard. After reversed phase LC, detection was performed under multiple reaction monitoring conditions in positive electrospray mode. Linearity ranged from 0.5 to 200 ng per WBC sample. Within-run and overall accuracy and precision ranged from 95.3 to 101.1% and from 1.6 to 4.7%, respectively. All validation parameters fulfilled international requirements. Conclusions: This method can be easily performed on small samples and provides reliable data, including in children and neonates. |
| Databáze: | OpenAIRE |
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