Involvement of membrane tubulin in erythrocyte deformability and blood pressure
| Autor: | Alexis N. Campetelli, Noelia E. Monesterolo, Juan Pié, Marcelo S. Vatta, Carlos A. Arce, Sandra Ingrid Hope, Cesar H. Casale, Marina R. Amaiden, Verónica S. Santander |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Paclitaxel Physiology medicine.medical_treatment Intraperitoneal injection Blood Pressure Cell membrane chemistry.chemical_compound Tubulin Erythrocyte Deformability Rats Inbred SHR Internal medicine otorhinolaryngologic diseases Internal Medicine Animals Humans Medicine Erythrocyte deformability Rats Wistar biology business.industry Nocodazole Cell Membrane Membrane Proteins Rats medicine.anatomical_structure Blood pressure Endocrinology Microscopy Fluorescence Membrane protein chemistry Decreased blood pressure Hypertension embryonic structures biology.protein Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of Hypertension. 30:1414-1422 |
| ISSN: | 0263-6352 |
| DOI: | 10.1097/hjh.0b013e328353b19a |
| Popis: | Objective To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and results Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a higher proportion of Mem-tub. Increased Mem-tub content in hypertensive patients was correlated with decreased erythrocyte deformability. Treatment of erythrocytes from normotensive individuals with taxol increased Mem-tub content and reduced deformability, whereas treatment of hypertensive patients erythrocytes with nocodazole had the opposite effect. In-vivo experiments with rats were performed to examine the possible relationship between Mem-tub content, erythrocyte deformability, and blood pressure. Spontaneously hypertensive rats (SHRs) showed lower erythrocyte deformability than normotensive Wistar rats. During the development of hypertension in SHR, tubulin in erythrocytes is translocated to the membrane, and this process is correlated with decreased deformability. In-vivo treatment (intraperitoneal injection) of SHR with nocodazole decreased Mem-tub content, increased erythrocyte deformability, and decreased blood pressure, whereas treatment of Wistar rats with taxol had the opposite effects. Conclusion These findings indicate that increased Mem-tub content contributes to reduced erythrocyte deformability in hypertensive animals. |
| Databáze: | OpenAIRE |
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