Secretory microRNA-29 expression in gingival crevicular fluid during orthodontic tooth movement

Autor: Grace Viana, Xiaofeng Zhou, Phimon Atsawasuwan, Yinghua Chen, Paul Lazari, Carlotta A. Evans
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Teeth
Tooth Movement Techniques
Molecular biology
Osteoclasts
Gene Expression
lcsh:Medicine
Centrifugation
Exosomes
Biochemistry
0302 clinical medicine
Animal Cells
Gene expression
Medicine and Health Sciences
lcsh:Science
Connective Tissue Cells
Multidisciplinary
medicine.diagnostic_test
Gingival Crevicular Fluid
Nucleic acids
RNA isolation
Separation Processes
Real-time polymerase chain reaction
Connective Tissue
Female
RNA extraction
Cellular Structures and Organelles
Anatomy
Cellular Types
Research Article
Oral Medicine
Orthodontics
Biomolecular isolation
Andrology
03 medical and health sciences
Extraction techniques
Dogs
Western blot
microRNA
medicine
Genetics
Animals
Humans
Vesicles
Liquid biopsy
Non-coding RNA
Bone
Biology and life sciences
business.industry
Gene Expression Profiling
lcsh:R
Liquid Biopsy
030206 dentistry
Cell Biology
Microvesicles
Gene regulation
Gene expression profiling
Research and analysis methods
MicroRNAs
030104 developmental biology
Biological Tissue
Molecular biology techniques
Jaw
RNA
lcsh:Q
business
Digestive System
Head
Ultracentrifugation
Biomarkers
Zdroj: PLoS ONE, Vol 13, Iss 3, p e0194238 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Secretory microRNAs (miRNAs) have been used increasingly as biomarkers for cancers, autoimmune diseases and inflammatory diseases. They are reported as being freely circulated or encapsulated in microvesicles such as exosomes. This study was performed to elucidate the presence of miRNAs with exosomes in human gingival crevicular fluid (GCF), and the expression profile of miRNA-29 during orthodontic tooth movement. Four healthy volunteer and fifteen orthodontic patients were enrolled in the study. Secretory miRNA in GCF was collected and analyzed using a bioanalyzer, realtime PCR and Western blot analysis. The expression profile of secretory miR-29 family in GCF was analyzed during the course of canine retraction for 6 weeks. The results demonstrated the presence of miRNAs in the GCF. After series of ultracentrifugation and RT-PCR array, exosome-depleted fractions and pellets were isolated and we found that secretory miRNAs were detected in both the exosome-associated fraction and the exosome-depleted supernatant fraction; however, the concentration of miRNAs was higher in the exosome-associated fraction than in the exosome-depleted fraction suggesting a close association between the secretory miRNAs and exosomes in GCF. We also demonstrated the increased expression profiles of miR-29 family during six weeks of orthodontic tooth movement in humans. Secretory miRNAs are present in GCF and secretory miRNA-29 family expression profiles increase during the tooth movement in humans. Secretory miRNA-29 in GCF could serve as potential biomarkers for periodontal remodeling.
Databáze: OpenAIRE
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