The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis
Autor: | Fangyan Yu, Kai S. Erdmann, Agnieszka Skowronek, Shruti Sharma |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
TRPP Cation Channels Endosome Green Fluorescent Proteins Vesicular Transport Proteins Endosomes Biology Ciliopathies Article Mice 03 medical and health sciences 0302 clinical medicine Protein Domains Antigens Neoplasm Intraflagellar transport Ciliogenesis Animals Humans Basal body Cilia Kidney Tubules Collecting RNA Small Interfering Binding Sites Microscopy Confocal Multidisciplinary Tumor Suppressor Proteins Cilium Intracellular Signaling Peptides and Proteins Transport protein Cell biology Gene Expression Regulation Neoplastic Protein Transport HEK293 Cells 030104 developmental biology 030217 neurology & neurosurgery Intraciliary transport HeLa Cells Signal Transduction |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep35399 |
Popis: | A primary cilium is present on most eukaryotic cells and represents a specialized organelle dedicated to signal transduction and mechanosensing. Defects in cilia function are the cause for several human diseases called ciliopathies. The serologically defined colon cancer antigen-3 (SDCCAG3) is a recently described novel endosomal protein mainly localized at early and recycling endosomes and interacting with several components of membrane trafficking pathways. Here we describe localization of SDCCAG3 to the basal body of primary cilia. Furthermore, we demonstrate that decreased expression levels of SDCCAG3 correlate with decreased ciliary length and a reduced percentage of ciliated cells. We show that SDCCAG3 interacts with the intraflagellar transport protein 88 (IFT88), a crucial component of ciliogenesis and intraciliary transport. Mapping experiments revealed that the N-terminus of SDCCAG3 mediates this interaction by binding to a region within IFT88 comprising several tetratricopeptide (TRP) repeats. Finally, we demonstrate that SDCCAG3 is important for ciliary localization of the membrane protein Polycystin-2, a protein playing an important role in the formation of polycystic kidney disease, but not for Rab8 another ciliary protein. Together these data suggest a novel role for SDCCAG3 in ciliogenesis and in localization of cargo to primary cilia. |
Databáze: | OpenAIRE |
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