Can Chlamydia abortus be transmitted by embryo transfer in goats?
| Autor: | C. Roux, Annie Rodolakis, Fabien Vorimore, J.F. Bruyas, Francis Fieni, M. Larrat, Karine Laroucau, J.L. Pellerin, S. Michaud, M. Oseikria |
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| Přispěvatelé: | Unité de sécurité sanitaire de la reproduction, PRES Université Nantes Angers Le Mans (UNAM), Ecole Nationale Vétérinaire Agroalimentaire et de l'Alimentation Nantes Atlantique (ONIRIS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Laboratoire de santé animale, Unité de zoonose bactérienne, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment FERTILIZED BOVINE EMBRYOS chlamydia Polymerase Chain Reaction law.invention IETS Food Animals law Caprine embryo Small Animals Zona pellucida Incubation Polymerase chain reaction MYCOPLASMA-BOVIS biology Goats Embryo C. abortus Embryo transfer 3. Good health medicine.anatomical_structure PCR embryonic structures transmission d'agents pathogènes DNA Bacterial animal structures chèvre transfert d'embryon PSITTACI INFECTION Andrology 03 medical and health sciences medicine Animals nannygoats HARDJO TYPE HARDJOBOVIS CHLAMYDOPHILA-ABORTUS pcr en temps réel Goat Diseases In vitro fertilisation [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Equine ENZOOTIC ABORTION Chlamydia Infections Embryo Mammalian biology.organism_classification Virology ENCEPHALITIS-VIRUS Chlamydophila abortus traitement antibiotique 030104 developmental biology SHEEP Animal Science and Zoology REPRODUCTIVE-TRACT IN-VITRO FERTILIZATION Bacteria |
| Zdroj: | Theriogenology Theriogenology, Elsevier, 2016, 86 (6), pp.1482-1488. ⟨10.1016/j.theriogenology.2016.05.006⟩ |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2016.05.006⟩ |
| Popis: | The objectives of this study were to determine (i) whether Chlamydia abortus would adhere to or penetrate the intact zona pellucida (ZP-intact) of early in vivo-derived caprine embryos, after in vitro infection; and (ii) the efficacy of the International Embryo Transfer Society (IETS) washing protocol for bovine embryos. Fifty-two ZP-intact embryos (8-16 cells), obtained from 14 donors were used in this experiment. The embryos were randomly divided into 12 batches. Nine batches (ZP-intact) of five embryos were incubated in a medium containing 4 x 10(7) Chlamydia/mL of AB7 strain. After incubation for 18 hours at 37 degrees C in an atmosphere of 5% CO2, the embryos were washed in batches in 10 successive baths of a phosphate buffer saline and 5% fetal calf serum solution in accordance with IETS guidelines. In parallel, three batches of ZP-intact embryos were used as controls by being subjected to similar procedures but without exposure to C. abortus. The 10 wash baths were collected separately and centrifuged for 1 hour at 13,000 x g. The washed embryos and the pellets of the 10 centrifuged wash baths were frozen at -20 degrees C before examination for evidence of C. abortus using polymerase chain reaction. C abortus DNA was found in all of the infected batches of ZP-intact embryos (9/9) after 10 successive washes. It was also detected in the 10th wash fluid for seven batches of embryos, whereas for the two other batches, the last positive wash bath was the eighth and the ninth, respectively. In contrast, none of the embryos or their washing fluids in the control batches were DNA positive. These results report that C abortus adheres to and/or penetrates the ZP of in vivo caprine embryos after in vitro infection, and that the standard washing protocol recommended by the IETS for bovine embryos, failed to remove it. The. persistence of these bacteria after washing makes the embryo a potential means of transmission of the bacterium during embryo transfer from infected donor goats to healthy recipients and/or their offspring. Nevertheless, the detection of C abortus DNA by polymerase chain reaction does not prove that the bacteria found was infectious. Further studies are required to investigate whether enzymatic and/or antibiotic treatment of caprine embryos infected by C abortus would eliminate the bacteria from the ZP. (C) 2016 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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