Immobilization of antimicrobial core-shell nanospheres onto silicone for prevention of Escherichia coli biofilm formation
| Autor: | Margarida M. Fernandes, Kristina Ivanova, Antonio Francesko, Tzanko Tzanov, Ernest Mendoza |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament d'Enginyeria Química, Universitat Politècnica de Catalunya. Departament de Física, Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Core-shell nanospheres Biopolímers Silicone functionalization Bioengineering 02 engineering and technology engineering.material medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound Silicone Enginyeria química [Àrees temàtiques de la UPC] Biopolymers medicine Escherichia coli Escherichia coli (Bacteri) Polydimethylsiloxane biology Epoxy-amine grafting Biofilm Antibiofilm strategies 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification 030104 developmental biology chemistry Biofilms engineering Surface modification Biopolymer 0210 nano-technology Bacteria Sonochemistry |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) |
| Popis: | Escherichia coli (E. coli) strains are among the most frequently isolated microorganisms in urinary tract infections able to colonize the surface of urinary catheters and form biofilms. These biofilms are highly resistant to antibiotics and host immune system, resulting in increased morbidity and mortality rates. Strategies to prevent biofilm development, especially via restricting the initial stages of bacteria attachment are therefore urgently needed. Herein, a common urinary catheter material – polydimethylsiloxane (PDMS) – was covalently functionalized with antibacterial aminocellulose nanospheres (ACNSs) using the epoxy/amine grafting chemistry. The PDMS surface was pre-activated with (3-glycidyloxypropyl)-triethoxysilane to introduce epoxy functionalities prior to immobilization of the intact ACNSs via its amino groups. The AC biopolymer was first sonochemically processed into NSs improving by up to 80% its potential to prevent the E. coli biofilm formation on a polystyrene surface. The silicone surface decorated with these NSs demonstrated efficient inhibition of E. coli biofilms, reducing the total biomass when compared with pristine silicone material. Therefore, the functionalization of silicone-based materials with ACNSs shows promise as potential platform for prevention of biofilm-associated infections caused by E. coli. |
| Databáze: | OpenAIRE |
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