CELL VIABILITY IN X-RAY IRRADIATED HUMAN LENS EPITHELIAL CELL (HLEC) CULTURE BY USING TRYPAN BLUE EXCLUSION TEST

Autor: Shivani Bapat, Paikrao, Rajesh Dehankar, Ksheersagar D D
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Journal of Evidence Based Medicine and Healthcare, Vol 2, Iss 60, Pp 9003-9008 (2015)
ISSN: 2349-2570
2349-2562
Popis: INTRODUCTION Cataract is one of major cause of blindness, 44% cases of blindness is due to cataract only. In developing countries such as India cataract is more common and it manifest earlier in life than the most developed countries of the world. Patients receiving therapeutic radiation may have developed cataracts as well as radiation retinopathy and optic nerve damage. In this study we are aimed to compare effects of X-ray irradiation on cell viability of HLEC (Human lens epithelial cell) culture by using trypan blue dye exclusion test against the unexposed control HLEC culture. Total 20 cataract patients from Dept. of Ophthalmology of Lata Mangeshkar Hospital, Nagpur were included in this study. The capsulorhexis sample from cataract surgery taken for study from eye OT. The capsulorhexis sample without any x-ray exposure taken as control. For culture of HLEC’s RPMI 1640 medium containing 10% fetal calf serum used. The HLEC cultures were X-ray irradiated with increasing count of skull limit parameters of range 150kV, 250mAs and 50mA for 5sec using GE® 650 M.A. X-ray machine. The Trypan blue dye exclusion test was used to analyze cell viability. The slides were analyzed using Olympus® BX51 Research microscope with oil immersion and 100 were observed per sample. The cell viability was calculated using Epi info® (version 6.0), as subject means with standard deviation. Statistical significance will be defined at P≤0.001. Out of 100 cases the 12 were female and 8 were male. Mean age of both male and female patients were 62.45±3.18(SD). A positive linear correlation was found (r:0.819, p
Databáze: OpenAIRE
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