Oral Administration of Inducible Nitric Oxide Synthase Inhibitors Reduces Nitric Oxide Synthesis but Has No Effect on the Severity of Experimental Colitis
| Autor: | C. Gannon, S. J. Kirk, G. R. Campbell, K R Gardiner, A. M. Armstrong |
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| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Arginine Administration Oral Nitric Oxide Guanidines Severity of Illness Index Inflammatory bowel disease Statistics Nonparametric Nitric oxide chemistry.chemical_compound Intestinal mucosa Reference Values Internal medicine medicine Citrulline Animals Enzyme Inhibitors Intestinal Mucosa Rats Wistar Nitrite Colitis Dose-Response Relationship Drug biology Gastroenterology medicine.disease Rats Nitric oxide synthase Disease Models Animal NG-Nitroarginine Methyl Ester Endocrinology Trinitrobenzenesulfonic Acid chemistry biology.protein Nitric Oxide Synthase |
| Zdroj: | Scandinavian Journal of Gastroenterology. 35:832-838 |
| ISSN: | 1502-7708 0036-5521 |
| Popis: | Increased concentrations of nitrate and nitrite (the breakdown products of nitric oxide) in the serum and faeces of patients with inflammatory bowel disease (IBD) suggests that increased synthesis of nitric oxide occurs in IBD. The aim of this study was to assess aminoguanidine (AMG), a selective inhibitor of inducible nitric oxide synthase, with regard to its effectiveness as a nitric oxide inhibitor and as a modulator of inflammation in trinitrobenzene sulfonic acid (TNBS)-induced colitis.Colitis was induced in Wistar rats. Selective (AMG) and non-selective (1-nitroso-arginine methyl ester (1-NAME)) inhibitors of nitric oxide synthase were given in the drinking water. Colonic citrulline and arginine concentrations were assessed using high-performance liquid chromatography. The severity of colitis was assessed by a macroscopic scoring system.Both 1-NAME and AMG successfully reduced nitric oxide synthesis. There was no evidence of substrate depletion in the colonic wall. Neither of the agents reduced the severity of colonic inflammation.Oral administration of nitric oxide synthase inhibitors reduced nitric oxide synthesis in the colonic wall. This study does not provide evidence to support a role for nitric oxide in the pathogenesis of colonic inflammation in TNBS colitis. |
| Databáze: | OpenAIRE |
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